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المؤلفJiang W.
المؤلفGriffanti G.
المؤلفTamimi F.
المؤلفMcKee M.D.
المؤلفNazhat S.N.
تاريخ الإتاحة2022-05-31T19:01:22Z
تاريخ النشر2020
اسم المنشورJournal of Structural Biology
المصدرScopus
المعرّفhttp://dx.doi.org/10.1016/j.jsb.2020.107592
معرّف المصادر الموحدhttp://hdl.handle.net/10576/31831
الملخصThe mineralized extracellular matrix of bone is an organic?inorganic nanocomposite consisting primarily of carbonated hydroxyapatite, fibrous type I collagen, noncollagenous proteins, proteoglycans, and diverse biomolecules such as pyrophosphate and citrate. While much is now known about the mineralization-regulating role of pyrophosphate, less is known about the function of citrate. In order to assess the effect of negatively charged citrate on collagen mineralization, citrate-functionalized, bone osteoid-mimicking dense collagen gels were exposed to simulated body fluid for up to 7 days to examine the multiscale evolution of intra- and interfibrillar collagen mineralization. Here, we show by increases in methylene blue staining that the net negative charge of collagen can be substantially augmented through citrate functionalization. Structural and compositional analyses by transmission and scanning electron microscopy (including X-ray microanalysis and electron diffraction), and atomic force microscopy, all demonstrated that citrate-functionalized collagen fibrils underwent extensive intrafibrillar mineralization within 12 h in simulated body fluid. Time-resolved, high-resolution transmission electron microscopy confirmed the temporal evolution of intrafibrillar mineralization of single collagen fibrils. Longer exposure to simulated body fluid resulted in additional interfibrillar mineralization, all through an amorphous-to-crystalline transformation towards apatite (assessed by X-ray diffraction and attenuated total reflection-Fourier-transform infrared spectroscopy). Calcium deposition assays indicated a citrate concentration-dependent temporal increase in mineralization, and micro-computed tomography confirmed that >80 vol% of the collagen in the gels was mineralized by day 7. In conclusion, citrate effectively induces mesoscale intra- and interfibrillar collagen mineralization, a finding that advances our understanding of the role of citrate in mineralized tissues
اللغةen
الناشرAcademic Press Inc.
الموضوعapatite
calcium
citric acid
collagen gel
methylene blue
citric acid
collagen type 1
hydroxyapatite
Article
atomic force microscopy
biomineralization
body fluid
chemical composition
chemical structure
collagen fibril
concentration (parameter)
controlled study
electron diffraction
extracellular matrix
Fourier transform infrared spectroscopy
micro-computed tomography
osteoid
priority journal
scanning electron microscopy
staining
transmission electron microscopy
X ray diffraction
X ray microanalysis
animal
biomimetics
bone
bone mineralization
gel
infrared spectroscopy
metabolism
physiology
procedures
rat
Animals
Apatites
Biomimetics
Bone and Bones
Calcification, Physiologic
Citric Acid
Collagen Type I
Durapatite
Extracellular Matrix
Gels
Microscopy, Electron, Scanning
Rats
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
X-Ray Microtomography
العنوانMultiscale structural evolution of citrate-triggered intrafibrillar and interfibrillar mineralization in dense collagen gels
النوعArticle
رقم العدد1
رقم المجلد212
dc.accessType Abstract Only


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