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AuthorZupin, Luisa
AuthorFontana, Francesco
AuthorClemente, Libera
AuthorBorelli, Violetta
AuthorRicci, Giuseppe
AuthorRuscio, Maurizio
AuthorCrovella, Sergio
Available date2022-11-02T07:26:25Z
Publication Date2022-09-27
Publication NameViruses
Identifierhttp://dx.doi.org/10.3390/v14102132
CitationZupin, L.; Fontana, F.; Clemente, L.; Borelli, V.; Ricci, G.; Ruscio, M.; Crovella, S. Optimization of Anti-SARS-CoV-2 Treatments Based on Curcumin, Used Alone or Employed as a Photosensitizer. Viruses 2022, 14, 2132. https://doi.org/10.3390/v14102132
URIhttp://hdl.handle.net/10576/35719
AbstractCurcumin, the bioactive compound of the spice , has already been reported as a potential COVID-19 adjuvant treatment due to its immunomodulatory and anti-inflammatory properties. In this study, SARS-CoV-2 was challenged with curcumin; moreover, curcumin was also coupled with laser light at 445 nm in a photodynamic therapy approach. Curcumin at a concentration of 10 μM, delivered to the virus prior to inoculation on cell culture, inhibited SARS-CoV-2 replication (reduction >99%) in Vero E6 cells, possibly due to disruption of the virion structure, as observed using the RNase protection assay. However, curcumin was not effective as a prophylactic treatment on already-infected Vero E6 cells. Notably, when curcumin was employed as a photosensitizer and blue laser light at 445 nm was delivered to a mix of curcumin/virus prior to the inoculation on the cells, virus inactivation was observed (>99%) using doses of curcumin that were not antiviral by themselves. Photodynamic therapy employing crude curcumin can be suggested as an antiviral option against SARS-CoV-2 infection.
SponsorThis research was funded by RC 15/2017 and 47/2020 from IRCCS Burlo Garofolo /Italian Ministry of Health and by the QUCG-CAS-22/23-499 Collaborative Grant from Qatar University.
Languageen
PublisherMDPI
SubjectSARS-CoV-2
Vero E6 cells
curcumin
in vitro infection
photodynamic therapy
TitleOptimization of Anti-SARS-CoV-2 Treatments Based on Curcumin, Used Alone or Employed as a Photosensitizer.
TypeArticle
Issue Number10
Volume Number14
ESSN1999-4915
dc.accessType Open Access


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