Show simple item record

AuthorSaadaoui, Imen
AuthorBounnit, Touria
AuthorMraiche, Fatima
AuthorJoseph, Jensa M.
AuthorCherif, Maroua
AuthorAl-Jabri, Hareb
Available date2023-02-19T06:35:44Z
Publication Date2022-01-01
Publication NameMolecular and Cellular Biochemistry
Identifierhttp://dx.doi.org/10.1007/s11010-022-04626-7
CitationSaadaoui, I., Bounnit, T., Mraiche, F. et al. Asterarcys quadricellulare (Chlorophyceae) protects H9c2 cardiomyoblasts from H2O2-induced oxidative stress. Mol Cell Biochem (2022). https://doi.org/10.1007/s11010-022-04626-7
ISSN03008177
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85145190197&origin=inward
URIhttp://hdl.handle.net/10576/40121
AbstractOxidative stress has recently been identified as an important mediator of cardiovascular diseases. The need to find efficient antioxidant molecules is essential in the disease’s prevention. Therefore, the present study aimed to evaluate the potential of microalgae bioactive in protecting H9c2 cardiomyoblasts from H2O2-induced oxidative stress. Four microalgal species were investigated for their antioxidant capacity. A qualitative assessment of oxidative stress in H9c2 cardiomyoblasts stained with DCFH-DA, treated with the highly active microalgae extracts, was performed. The protein expression of total caspase-3 was also examined to investigate whether the extract protects H9c2 cardimyoblasts from H2O2-induced apoptosis. High antioxidant activity was observed for the hexanoic extracts after 10 days of cultivation. Asterarcys quadricellulare exhibited the highest antioxidant capacity of 110.59 ± 1.75 mg TE g−1 dry weight and was tested against H9c2 cardiomyoblasts, which were initially subjected to H2O2-induced oxidative stress. This hexanoic extract protected against H2O2 induced oxidative stress with a similar scavenging capacity as N-Acetylcysteine. Furthermore, total caspase-3 was increased following treatment with the hexanoic extract, suggesting that A. quadricellulare also had anti-apoptotic properties. The outcome of our study highlighted the possible use of the local A. quadricellulare strain QUCCCM10 as a natural, safe, and efficient antioxidant to prevent cardiovascular diseases.
SponsorOpen Access funding provided by the Qatar National Library. This report was made possible by the NPRP award [NPRP8-1087-1-207] from the Qatar National Research Fund (a member of The Qatar Foundation) and Start Up project [QUSG-CAS-CSD-14/15-9]. The statements made herein are solely the responsibility of the authors.
Languageen
PublisherSpringer
SubjectAntioxidant
Asterarcys quadricellulare
Cardiovascular diseases
Caspase-3
High value products
Reactive Oxygen Species
TitleAsterarcys quadricellulare (Chlorophyceae) protects H9c2 cardiomyoblasts from H2O2-induced oxidative stress
TypeArticle
ESSN1573-4919
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record