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المؤلفAl-Asmakh, Maha
المؤلفStukenborg, Jan-Bernd
المؤلفReda, Ahmed
المؤلفAnuar, Farhana
المؤلفStrand, Mona-Lisa
المؤلفHedin, Lars
المؤلفPettersson, Sven
المؤلفSoder, Olle
تاريخ الإتاحة2016-02-22T09:04:54Z
تاريخ النشر2014-08
اسم المنشورPLoS ONE
المصدرScopus
الاقتباسAl-Asmakh, M., Stukenborg, J.-B., Reda, A., Anuar, F., Strand, M.-L., Hedin, L., Pettersson, S., S?der, O. "The gut microbiota and developmental programming of the testis in mice", (2014) PLoS ONE, 9 (8), art. no. e103809.
الرقم المعياري الدولي للكتاب1932-6203
معرّف المصادر الموحدhttp://dx.doi.org/10.1371/journal.pone.0103809
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4172
الملخصNutrients and environmental chemicals, including endocrine disruptors, have been incriminated in the current increase in male reproductive dysfunction, but the underlying mechanisms remain unknown. The gastrointestinal tract represents the largest surface area exposed to our environment and thereby plays a key role in connection with exposure of internal organs to exogenous factors. In this context the gut microbiome (all bacteria and their metabolites) have been shown to be important contributors to body physiology including metabolism, cognitive functions and immunity. Pivotal to male reproduction is a proper development of the testis, including the formation of the blood-testis barrier (BTB) that encapsulates and protects germ cells from stress induced environmental cues, e.g. pathogenic organisms and xenobiotics. Here we used specific pathogen free (SPF) mice and germ-free (GF) mice to explore whether gut microbiota and/or their metabolites can influence testis development and regulation of BTB. Lumen formation in the seminiferous tubules, which coincides with the development of the BTB was delayed in the testes of GF mice at 16 days postpartum. In addition, perfusion experiments (Evans blue) demonstrated increased BTB permeability in these same mice. Reduced expressions of occludin, ZO-2 and E-cadherin in GF testis suggested that the microbiota modulated BTB permeability by regulation of cell-cell adhesion. Interestingly, exposure of GF mice to Clostridium Tyrobutyricum (CBUT), which secrete high levels of butyrate, restored the integrity of the BTB and normalized the levels of cell adhesion proteins. Moreover, the GF mice exhibited lower serum levels of gonadotropins (LH and FSH) than the SPF group. In addition, the intratesticular content of testosterone was lower in GF compared to SPF or CBUT animals. Thus, the gut microbiome can modulate the permeability of the BTB and might play a role in the regulation of endocrine functions of the testis.
اللغةen
الناشرPublic Library of Science
الموضوعBlood-Testis Barrier
Cell Adhesion
Fertility
Mice, Inbred C57BL
Mice, Inbred Strains
Microbiota
Specific Pathogen-Free Organisms
العنوانThe gut microbiota and developmental programming of the testis in mice
النوعArticle
رقم العدد8
رقم المجلد9
dc.accessType Open Access


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