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AuthorRahman, Aura
AuthorStyczynski, Ashley
AuthorKhaleque, Abdul
AuthorHossain, Sakib Abrar
AuthorSadique, Abdus
AuthorHossain, Arman
AuthorJain, Mukesh
AuthorTabassum, Syeda Naushin
AuthorKhan, Fahad
AuthorBhuiyan, Mohammad Sami Salman
AuthorAlam, Jahidul
AuthorKhandakar, Amith
AuthorKamruzzaman, Mohammad
AuthorAhsan, Chowdhury Rafiqul
AuthorKashem, Saad Bin Abul
AuthorChowdhury, Muhammad E. H.
AuthorHossain, Maqsud
Available date2023-04-17T06:57:41Z
Publication Date2022
Publication NameBMC Genomics
ResourceScopus
URIhttp://dx.doi.org/10.1186/s12864-022-08991-x
URIhttp://hdl.handle.net/10576/41932
AbstractBackground: Acinetobacter calcoaceticus-A. baumannii (ACB) complex pathogens are known for their prevalence in nosocomial infections and extensive antimicrobial resistance (AMR) capabilities. While genomic studies worldwide have elucidated the genetic context of antibiotic resistance in major international clones (ICs) of clinical Acinetobacter spp., not much information is available from Bangladesh. In this study, we analysed the AMR profiles of 63 ACB complex strains collected from Dhaka, Bangladesh. Following this, we generated draft genomes of 15 of these strains to understand the prevalence and genomic environments of AMR, virulence and mobilization associated genes in different Acinetobacter clones. Results: Around 84% (n = 53) of the strains were extensively drug resistant (XDR) with two showing pan-drug resistance. Draft genomes generated for 15 strains confirmed 14 to be A. baumannii while one was A. nosocomialis. Most A. baumannii genomes fell under three clonal complexes (CCs): the globally dominant CC1 and CC2, and CC10; one strain had a novel sequence type (ST). AMR phenotype-genotype agreement was observed and the genomes contained various beta-lactamase genes including blaOXA-23 (n = 12), blaOXA-66 (n = 6), and blaNDM-1 (n = 3). All genomes displayed roughly similar virulomes, however some virulence genes such as the Acinetobactin bauA and the type IV pilus gene pilA displayed high genetic variability. CC2 strains carried highest levels of plasmidic gene content and possessed conjugative elements carrying AMR genes, virulence factors and insertion sequences. Conclusion: This study presents the first comparative genomic analysis of XDR clinical Acinetobacter spp. from Bangladesh. It highlights the prevalence of different classes of beta-lactamases, mobilome-derived heterogeneity in genetic architecture and virulence gene variability in prominent Acinetobacter clonal complexes in the country. The findings of this study would be valuable in understanding the genomic epidemiology of A. baumannii clones and their association with closely related pathogenic species like A. nosocomialis in Bangladesh. 2022, The Author(s).
SponsorThis work was funded by North South University Conference Travel and Research Grants (NSU CTRG) Committee under the grant number: NSU-RP-18-042.
Languageen
PublisherBioMed Central Ltd
SubjectAcinetobacter baumannii
Acinetobacter nosocomialis
Clonal complex
Comparative genomics
WGS
TitleGenomic landscape of prominent XDR Acinetobacter clonal complexes from Dhaka, Bangladesh
TypeArticle
Issue Number1
Volume Number23
dc.accessType Open Access


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