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AuthorYammine, Jina
AuthorGharsallaoui, Adem
AuthorKaram, Layal
AuthorIsmail, Ali
AuthorFadel, Alexandre
AuthorChihib, Nour Eddine
Available date2023-05-10T04:04:28Z
Publication Date2022-12-01
Publication NameBiofilm
Identifierhttp://dx.doi.org/10.1016/j.bioflm.2022.100094
CitationYammine J, Gharsallaoui A, Karam L, Ismail A, Fadel A, Chihib NE. Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol. Biofilm. 2022 Nov 26;4:100094. doi: 10.1016/j.bioflm.2022.100094.
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85145572954&origin=inward
URIhttp://hdl.handle.net/10576/42522
AbstractIn food industries, microbial contaminations are difficult to control due to the recurrent formation of biofilms that hinders antimicrobials penetration and efficiency. An understanding of Salmonella Enteritidis biofilms behavior under flow conditions is a key to develop efficient preventive and control strategies. S. Enteritidis biofilms displayed 5.96, 6.28 and 6.80 log CFU cm−2 under 0.006 cm s−1, 0.045 cm s−1, and 0.087 cm s−1 flow velocities, respectively. Biofilms exposed to higher nutrient conditions under greater flow rates, induced significantly more biofilm biomass. To control biofilms, the disinfection efficiency of thymol (THY) was assessed under dynamic conditions by encapsulation it into two types of nanocapsules: monolayer (ML) nanocapsules prepared with a single carrier material (maltodextrin), and layer-by-layer (LBL) nanocapsules prepared by combining two carrier materials (maltodextrin and pectin). A combined mixture of ML and LBL nanocapsules at ½ their minimal inhibitory concentrations induced 99.99% eradication of biofilms developed under the highest flow conditions, after 5 h. ML nanocapsules decreased significantly bacterial counts during the first 0.5 h, while LBL nanocapsules eliminated the remaining bacterial cells and ensured a protection from bacterial contamination for up to 5 h by releasing THY in a sustained manner over time due to the thicker shell wall structure.
SponsorThe authors are grateful to Dr. Heni Dallagi and Laurent Wauquier for their valuable technical assistance. The Chevreul Institute is also thanked for its help in the development of this work through the ARCHI-CM project supported by the “Ministère de l’Enseignement Supérieur de la Recherche et de l’Innovation”, the region “Hauts-de-France”, the ERDF program of the European Union and the “Métropole Européenne de Lille.
Languageen
PublisherElsevier
SubjectDisinfection
Dynamic biofilm
Hydrodynamic conditions
Nanoencapsulation
Salmonella enteritidis
Thymol
TitleDynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol
TypeArticle
Volume Number4
ESSN2590-2075
dc.accessType Full Text


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