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AuthorMechmechani, Samah
AuthorGharsallaoui, Adem
AuthorKaram, Layal
AuthorEL Omari, Khaled
AuthorFadel, Alexandre
AuthorHamze, Monzer
AuthorChihib, Nour Eddine
Available date2023-05-25T10:34:35Z
Publication Date2023-01-06
Publication NameMicroorganisms
Identifierhttp://dx.doi.org/10.3390/microorganisms11010143
CitationMechmechani, S., Gharsallaoui, A., Karam, L., EL Omari, K., Fadel, A., Hamze, M., & Chihib, N. E. (2023). Pepsin and Trypsin Treatment Combined with Carvacrol: An Efficient Strategy to Fight Pseudomonas aeruginosa and Enterococcus faecalis Biofilms. Microorganisms, 11(1), 143.
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85146823103&origin=inward
URIhttp://hdl.handle.net/10576/43470
AbstractBiofilms consist of microbial communities enclosed in a self-produced extracellular matrix which is mainly responsible of biofilm virulence. Targeting this matrix could be an effective strategy to control biofilms. In this work, we examined the efficacy of two proteolytic enzymes, pepsin and trypsin, to degrade P. aeruginosa and E. faecalis biofilms and their synergistic effect when combined with carvacrol. The minimum dispersive concentrations (MDCs) and the contact times of enzymes, as well as the minimal inhibitory concentrations (MICs) and contact times of carvacrol, were determined against biofilms grown on polystyrene surfaces. For biofilms grown on stainless steel surfaces, the combined pepsin or trypsin with carvacrol treatment showed more significant reduction of both biofilms compared with carvacrol treatment alone. This reduction was more substantial after sequential treatment of both enzymes, followed by carvacrol with the greatest reduction of 4.7 log CFU mL−1 (p < 0.05) for P. aeruginosa biofilm and 3.3 log CFU mL−1 (p < 0.05) for E. faecalis biofilm. Such improved efficiency was also obvious in the epifluorescence microscopy analysis. These findings demonstrate that the combined effect of the protease-dispersing activity and the carvacrol antimicrobial activity could be a prospective approach for controlling P. aeruginosa and E. faecalis biofilms.
SponsorThis work was supported by the University of Lille, CNRS, INRAE, Centrale Lille, UMET-Unité Matériaux et Transformations under Grant [UMR 8207].
Languageen
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
Subjectbiofilm
carvacrol
Enterococcus faecalis
pepsin
Pseudomonas aeruginosa
trypsin
TitlePepsin and Trypsin Treatment Combined with Carvacrol: An Efficient Strategy to Fight Pseudomonas aeruginosa and Enterococcus faecalis Biofilms
TypeArticle
Issue Number1
Volume Number11
ESSN2076-2607
dc.accessType Open Access


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