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AuthorRamadoss, Ramya
AuthorAl-Shukri, Moza
AuthorShomar, Basem
AuthorIlyin, Valentin Alekseevich
AuthorVincent, Annette Shoba
Available date2023-05-29T06:55:10Z
Publication Date2022-05-31
Publication NameScientific Reports
Identifierhttp://dx.doi.org/10.1038/s41598-022-13171-8
CitationRamadoss, R., Al-Shukri, M., Shomar, B., Ilyin, V. A., & Vincent, A. S. (2022). Substantiation of propitious “Enzybiotic” from two novel bacteriophages isolated from a wastewater treatment plant in Qatar. Scientific Reports, 12(1), 9093.
ISSN2045-2322
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85131012236&origin=inward
URIhttp://hdl.handle.net/10576/43527
AbstractLysin of bacteriophages isolated from a particular ecosystem could be inducted as a bio-controlling tool against the inhabiting pathogenic bacterial strains. Our study aims at both experimental and computational characterization of the identical lysin gene product inherent in the genomes of two novel Myoviridae bacteriophages, Escherichia Phage C600M2 (GenBank accession number OK040807, Protein ID: UCJ01465) and Escherichia Phage CL1 (GenBank Genome accession number OK040806.1, Protein ID: UCJ01321) isolated from wastewater collected from the main water treatment plant in Qatar. The lysin protein, evinced to be a globular N-acetyl-muramidase with intrinsic “cd00737: endolysin_autolysin” domain, was further expressed and purified to be experimentally validated by turbidimetric assay for its utility as an anti-bacterial agent. Comprehensive computational analysis revealed that the scrutinized lysin protein shared 85–98% sequence identity with 61 bacteriophages, all native to wastewater allied environments. Despite varied Host Recognition Components encoded in their genomes, the similitude of lysins, suggests its apparent significance in host–pathogen interactions endemic to wastewater environment. The present study substantiates the identical lysin from Escherichia Phage C600M2 and Escherichia Phage CL1 as propitious “enzybiotic”, a hybrid term to describe enzymes analogous to anti-biotics to combat antibiotic-resistant bacteria by in silico analysis and subsequent experimental validation.
SponsorThis publication was made possible by NPRP 10-0119-170197 from the Qatar National Research Fund (a member of Qatar Foundation).
Languageen
PublisherSpringer Nature
Subjectwater management
enzybiotic
Water Purification
TitleSubstantiation of propitious “Enzybiotic” from two novel bacteriophages isolated from a wastewater treatment plant in Qatar
TypeArticle
Issue Number1
Volume Number12
dc.accessType Open Access


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