Show simple item record

AuthorIslam, Z.
AuthorAli, M.H.
AuthorPopelka, A.
AuthorMall, R.
AuthorUllah, E.
AuthorPonraj, J.
AuthorKolatkar, P.R.
Available date2023-10-09T09:58:53Z
Publication Date2021
Publication NameJournal of Biomolecular Structure and Dynamics
ResourceScopus
URIhttp://dx.doi.org/10.1080/07391102.2020.1734091
URIhttp://hdl.handle.net/10576/48365
AbstractAmyloid fibrillation is the root cause of several neuro as well as non-neurological disorders. Understanding the molecular basis of amyloid aggregate formation is crucial for deciphering various neurodegenerative diseases. In our study, we have examined the lysozyme fibrillation process using nano-infrared spectroscopy (nanoIR). NanoIR enabled us to investigate both structural and chemical characteristics of lysozyme fibrillar species concurrently. The spectroscopic results indicate that lysozyme transformed into a fibrillar structure having mainly parallel ?-sheets, with almost no antiparallel ?-sheets. Features such as protein stiffness have a good correlation with obtained secondary structural information showing the state of the protein within the fibrillation state. The structural and chemical details were compared with transmission electron microscopy (TEM) and circular dichroism (CD). We have utilized nanoIR and measured infrared spectra to characterize lysozyme amyloid fibril structures in terms of morphology, molecular structure, secondary structure content, stability, and size of the cross-? core. We have shown that the use of nanoIR can complement other biophysical studies to analyze the aggregation process and is particularly useful for studying proteins involved in aggregation to help in designing molecules against amyloid aggregation. Specifically, the nanoIR spectra afford higher resolution information and a characteristic fingerprint for determining states of aggregation. Communicated by Ramaswamy H. Sarma. 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
SponsorP. R. K and Z. I. were supported by Qatar Biomedical Research Institute under the award IGP2 (2014-004). The publication of this article was funded by the Qatar National Library.
Languageen
PublisherTaylor and Francis Ltd.
Subjectamyloid fibrils
lysozyme
nanoIR
neurodegenerative diseases
Protein aggregation
TitleProbing the fibrillation of lysozyme by nanoscale-infrared spectroscopy
TypeArticle
Pagination1481-1490
Issue Number4
Volume Number39
dc.accessType Abstract Only


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record