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AuthorAziz, Khan
AuthorJie, Zheng
AuthorXiangjun, Kong
AuthorUllah, Najeeb
AuthorShort, Aidan W.
AuthorDiao, Yong
AuthorZhou, Ruiyang
AuthorXiong, You-Cai
Available date2023-10-11T06:39:22Z
Publication Date2023-03-05
Publication NameJournal of Hazardous Materials
Identifierhttp://dx.doi.org/10.1016/j.jhazmat.2022.130530
ISSN03043894
URIhttps://www.sciencedirect.com/science/article/pii/S030438942202324X
URIhttp://hdl.handle.net/10576/48422
AbstractMelatonin, a plant/animal origin hormone, regulates plant response to abiotic stresses by protecting them from oxidative damage. This study identified physiochemical and molecular mechanism of melatonin-induced cadmium (Cd) stress tolerance and detoxification in cotton seedlings. Cotton seedlings, with or without melatonin (15 µM) pretreatment, were subjected to Cd (100 µM) stress in a hydroponic medium for eight days. We found that higher cellular Cd accumulation in leaf tissues significantly inhibited the growth and physiology of cotton seedlings. In contrast, melatonin-treated seedlings maintained leaf photosynthetic capacity, producing relatively higher fresh (17.4%) and dry (19.3%) weights than non-melatonin-treated plants under Cd-contaminated environments. The improved growth and leaf functioning were strongly linked with the melatonin-induced repression of Cd transporter genes (LOC107894197, LOC107955631, LOC107899273) in roots. Thus, melatonin induced downregulation of the Cd transporter genes further inhibited Cd ion transport towards leaf tissues. This suggests that the differentially expressed transporter genes (DEG) are key drivers of the melatonin-mediated regulation of Cd transportation and sequestration in cotton. Melatonin also protected cotton seedlings from Cd-induced oxidative injury by reducing tissues malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels and increasing the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) enzymes. Transcriptomic analysis revealed that melatonin activated mitogen-activated protein kinase (MAPK) signaling pathways to simulate stomatal adjustment and photosynthesis in Cd-stressed leaves. Further, melatonin protects intercellular organs, particularly ribosomes, from Cd-induced oxidative damage by promoting ribosomal biosynthesis and improving translational efficiency. The findings elucidated the molecular basis of melatonin-mediated Cd stress tolerance in plants and provided a key for the effective strategy of Cd accumulation in cotton.
SponsorThis work was financially supported by National Natural Science Foundation of China (32161143012), Hainan Provincial Natural Science Foundation Joint Project of China (2021JJLH0032) and Hainan Yazhou Bay Seed Laboratory Program Postdoctoral Project (Grant B22Y10205).
Languageen
PublisherElsevier
SubjectMelatonin, auxin pathway, mitogen-activated protein kinase
Gene expression
Antioxidants
Leaf gas exchange
Cadmium sequestration
TitlePre treatment of melatonin rescues cotton seedlings from cadmium toxicity by regulating key physio-biochemical and molecular pathways
TypeArticle
Volume Number445
ESSN1873-3336
dc.accessType Open Access


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