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المؤلفElleuch, Jihen
المؤلفJaoua, Samir
المؤلفGinibre, Carole
المؤلفChandre, Fabrice
المؤلفTounsi, Slim
المؤلفZghal, Raida Z.
تاريخ الإتاحة2017-02-19T08:28:35Z
تاريخ النشر2016-03-29
اسم المنشورPest Management Science
المعرّفhttp://dx.doi.org/10.1002/ps.4261
الاقتباسElleuch, J., Jaoua, S., Ginibre, C., Chandre, F., Tounsi, S. and Zghal, R. Z. (2016), Toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa. Pest. Manag. Sci., 72: 2240–2246.
الرقم المعياري الدولي للكتاب1526-498X
معرّف المصادر الموحدhttp://hdl.handle.net/10576/5276
الملخصBACKGROUND Bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. Improving their efficacy is of great benefit. Here, an improvement in Cry2Aa δ-endotoxin toxicity was attempted via a cry gene over expression system using P20 from B. thuringiensis israelensis. RESULTS The coexpression of Cry2Aa with P20 resulted in a seven fold increase in its production yield in B. thuringiensis. Generated crystals proved to be significantly more toxic (505.207 µg g−1, 1.99 mg L−1 and 1.49 mg L−1) than the P20-lacking control (720.78 µg g−1, 705.69 mg L−1 and 508.51 mg L−1) against Ephestia kuehniella, Aedes aegypti and Culex pipiens larvae respectively. In vitro, processing experiments revealed a P20-mediated protection of Cry2Aa against degradation under larval gut conditions. Thus, P20 could promote the maintenance of a tightly packaged conformation of Cry2Aa toxins in the larval midgut upon correct activation and binding to its membrane receptors. CONCLUSION Based on their resistance against excessive proteolysis, Cry2Aa δ-endotoxins, produced in the presence of P20, could be considered as a successful control agent for E. kuehniella and an effective alternative for mosquito control, implying its possible exploitation in pest management programmes. © 2016 Society of Chemical Industry
راعي المشروعMinistry of Higher Education and Scientific Research
اللغةen
الناشرWiley
الموضوعP20 helper protein
Cry2Aa toxin
larvicidal activity improvement
protection against excessive proteolysis
العنوانToxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa
النوعArticle
الصفحات2240-2246
رقم العدد12
رقم المجلد72
ESSN1526-4998
dc.accessType Abstract Only


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