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AuthorElleuch, Jihen
AuthorJaoua, Samir
AuthorGinibre, Carole
AuthorChandre, Fabrice
AuthorTounsi, Slim
AuthorZghal, Raida Z.
Available date2017-02-19T08:28:35Z
Publication Date2016-03-29
Publication NamePest Management Science
Identifierhttp://dx.doi.org/10.1002/ps.4261
CitationElleuch, J., Jaoua, S., Ginibre, C., Chandre, F., Tounsi, S. and Zghal, R. Z. (2016), Toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa. Pest. Manag. Sci., 72: 2240–2246.
ISSN1526-498X
URIhttp://hdl.handle.net/10576/5276
AbstractBACKGROUND Bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. Improving their efficacy is of great benefit. Here, an improvement in Cry2Aa δ-endotoxin toxicity was attempted via a cry gene over expression system using P20 from B. thuringiensis israelensis. RESULTS The coexpression of Cry2Aa with P20 resulted in a seven fold increase in its production yield in B. thuringiensis. Generated crystals proved to be significantly more toxic (505.207 µg g−1, 1.99 mg L−1 and 1.49 mg L−1) than the P20-lacking control (720.78 µg g−1, 705.69 mg L−1 and 508.51 mg L−1) against Ephestia kuehniella, Aedes aegypti and Culex pipiens larvae respectively. In vitro, processing experiments revealed a P20-mediated protection of Cry2Aa against degradation under larval gut conditions. Thus, P20 could promote the maintenance of a tightly packaged conformation of Cry2Aa toxins in the larval midgut upon correct activation and binding to its membrane receptors. CONCLUSION Based on their resistance against excessive proteolysis, Cry2Aa δ-endotoxins, produced in the presence of P20, could be considered as a successful control agent for E. kuehniella and an effective alternative for mosquito control, implying its possible exploitation in pest management programmes. © 2016 Society of Chemical Industry
SponsorMinistry of Higher Education and Scientific Research
Languageen
PublisherWiley
SubjectP20 helper protein
Cry2Aa toxin
larvicidal activity improvement
protection against excessive proteolysis
TitleToxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa
TypeArticle
Pagination2240-2246
Issue Number12
Volume Number72
ESSN1526-4998
dc.accessType Abstract Only


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