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AuthorRakib, Fazle
AuthorAl-Saad, Khalid
AuthorUstaoglu, Sebnem G.
AuthorUllah, Ehsan
AuthorMall, Raghvendra
AuthorThompson, Richard
AuthorAbdelalim, Essam M.
AuthorAhmed, Tariq
AuthorSevercan, Feride
AuthorAli, Mohamed H. M.
Available date2024-04-29T07:07:47Z
Publication Date2021
Publication NameBrain Sciences
ResourceScopus
ISSN20763425
URIhttp://dx.doi.org/10.3390/brainsci11070918
URIhttp://hdl.handle.net/10576/54414
AbstractTraumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.
SponsorFunding: This study was made possible by a NPRP Award [7-1648-3-420-4] from the Qatar National Research Fund (a member of The Qatar Foundation). The statements made herein are solely the responsibility of the authors.
Languageen
PublisherMDPI AG
SubjectBiochemical analysis and immunohis-tochemistry
Fourier transform infrared
Traumatic brain injury
TitleFourier transform infrared imaging-A novel approach to monitor bio molecular changes in subacute mild traumatic brain injury
TypeArticle
Issue Number7
Volume Number11
dc.accessType Open Access


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