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المؤلفDa'as, Sahar I
المؤلفThanassoulas, Angelos
المؤلفCalver, Brian L
المؤلفBeck, Konrad
المؤلفSalem, Rola
المؤلفSaleh, Alaaeldin
المؤلفKontogianni, Iris
المؤلفAl-Maraghi, Ali
المؤلفNasrallah, Gheyath K
المؤلفSafieh-Garabedian, Bared
المؤلفToft, Egon
المؤلفNounesis, George
المؤلفLai, F Anthony
المؤلفNomikos, Michail
تاريخ الإتاحة2019-05-13T10:11:37Z
تاريخ النشر2019-04-01
اسم المنشورAnnals of the New York Academy of Sciences
المعرّفhttp://dx.doi.org/10.1111/nyas.14033
الاقتباسDa'as, Sahar I. et. al. Arrhythmogenic calmodulin E105A mutation alters cardiac RyR2 regulation leading to cardiac dysfunction in zebrafish, Annals of the New York Academy of Sciences. Wiley
الرقم المعياري الدولي للكتاب0077-8923
معرّف المصادر الموحدhttp://hdl.handle.net/10576/11542
الملخصCalmodulin (CaM) is a universal calcium (Ca )-binding messenger that regulates many vital cellular events. In cardiac muscle, CaM associates with ryanodine receptor 2 (RyR2) and regulates excitation-contraction coupling. Mutations in human genes CALM1, CALM2, and CALM3 have been associated with life-threatening heart disorders, such as long QT syndrome (LQTS) and catecholaminergic polymorphic ventricular tachycardia. A novel de novo LQTS-associated missense CaM mutation (E105A) was recently identified in a 6-year-old boy, who experienced an aborted first episode of cardiac arrest. Herein, we report the first molecular characterization of the CaM E105A mutation. Expression of the CaM E105A mutant in zebrafish embryos resulted in cardiac arrhythmia and increased heart rate, suggestive of ventricular tachycardia. In vitro biophysical and biochemical analysis revealed that E105A confers a deleterious effect on protein stability and a reduced Ca -binding affinity due to loss of cooperativity. Finally, the CaM E105A mutation resulted in reduced CaM-RyR2 interaction and defective modulation of ryanodine binding. Our findings suggest that the CaM E105A mutation dysregulates normal cardiac function by a complex mechanism involving alterations in both CaM-Ca and CaM-RyR2 interactions.
راعي المشروعM.N. was supported by a QU internal Grant “QUST‐CMED‐SPR‐2017‐8.” A.T. was supported from the State Scholarship Foundation (IKY) through the “IKY Fellowships of Excellence for Post‐Doctoral Research Program” MIS‐5001512.
اللغةen
الناشرWiley
الموضوعarrhythmia
calcium
calmodulin
long QT syndrome
ryanodine receptor 2
zebrafish
العنوانArrhythmogenic calmodulin E105A mutation alters cardiac RyR2 regulation leading to cardiac dysfunction in zebrafish
النوعArticle
ESSN1749-6632
dc.accessType Abstract Only


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