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المؤلفSultan, Ahmed
المؤلفYang, Keun-Hang Susan
المؤلفIsaev, Dmitro
المؤلفElNebrisi, Eslam
المؤلفSyed, Nurulain
المؤلفKhan, Nadia
المؤلفHowarth, Christopher F.
المؤلفSadek, Bassem
المؤلفOzad, Murat
تاريخ الإتاحة2020-09-20T08:35:37Z
تاريخ النشر2017
اسم المنشورToxicology
المصدرScopus
الرقم المعياري الدولي للكتاب0300483X
معرّف المصادر الموحدhttp://dx.doi.org/10.1016/j.tox.2017.04.005
معرّف المصادر الموحدhttp://hdl.handle.net/10576/16172
الملخصEffects of thujone, a major ingredient of absinthe, wormwood oil and some herbal medicines, were tested on the function of 7 subunit of the human nicotinic acetylcholine (7 nACh) receptor expressed in Xenopus oocytes using the two-electrode voltage-clamp technique. Thujone reversibly inhibited ACh (100 M)-induced currents with an IC50 value of 24.7 M. The effect of thujone was not dependent on the membrane potential and did not involve Ca2+-dependent Cl channels expressed endogenously in oocytes. Inhibition by thujone was not reversed by increasing ACh concentrations. Moreover, specific binding of [125I] -bungarotoxin was not altered by thujone. Further experiments in SH-EP1 cells expressing human 7 nACh receptor indicated that thujone suppressed choline induced Ca2+ transients in a concentration-dependent manner. In rat hippocampal CA3-dentate gyrus synapses, nicotine-induced enhancement of long-term potentiation was also inhibited by thujone. Furthermore, the results observed in in-vivo one-trial passive avoidance paradigm show that thujone (1.25 mg/kg, i.p.) significantly impaired nicotine-induced enhancement of learning and memory in Wistar rats. Collectively, our results indicate that thujone inhibits the function of the 7-nACh receptor and impairs cellular and behavioral correlates of cholinergic modulation of learning and memory.
اللغةen
الناشرElsevier Ireland Ltd
الموضوعAbsinthe
memory
Nicotinic receptors
passive avoidance paradigm
Thujone
Xenopus oocyte
العنوانThujone inhibits the function of 7-nicotinic acetylcholine receptors and impairs nicotine-induced memory enhancement in one-trial passive avoidance paradigm
النوعArticle
الصفحات23-32
رقم المجلد384


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