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المؤلفBlibech, Imen
المؤلفKsantini, Mohieddine
المؤلفChaieb, Ikbal
المؤلفJlassi, Brahim
المؤلفRhouma, Ali
المؤلفJaoua, Samir
المؤلفAifa, Sami
تاريخ الإتاحة2015-12-27T08:37:33Z
تاريخ النشر2012-01
اسم المنشورCrop Protection
المعرّفhttp://dx.doi.org/10.1016/j.cropro.2011.09.020
الاقتباس"Isolation of entomopathogenic Bacillus from a biodynamic olive farm and their pathogenicity to lepidopteran and coleopteran insect pests", Blibech, Imen; Ksantini, Mohieddine; Chaieb, Ikbal; Jlassi, Brahim; Rhouma, Ali, et al. (2012), Crop Protection, vol. 31(1):p. 72-77
الرقم المعياري الدولي للكتاب0261-2194
معرّف المصادر الموحدhttp://hdl.handle.net/10576/3990
الملخصThe occurrence of Bacillus entomopathogenic bacteria on a Tunisian biodynamic farm was determined by examining 75 samples from olive tree (Olea europaea L.) habitats. A total of 40 Bacillus isolates were characterized according to their phenotypic, physiological and biochemical parameters. Isolates of the species Bacillus subtilis, Bacillus mycoides, Brevibacillus brevis, Paenibacillus polymyxa, Bacillus licheniformis, Bacillus sp. (1), Bacillus sp. (2) and a standard strain Btk HD-1 were used separately in feeding bioassays on fresh artificial diet against larvae of lepidopterans Prays oleae (Bernard) and Palpita unionalis (Hübner) and coleopterans Hylesinus oleiperda (F.) and Phloeotribus scarabaeoides (Bernard), which are olive tree pests. Larvae were successfully reared on an artificial diet with 25 g powdered olive tree leaves. Compared to the control data, only Btk and the isolates of B. licheniformis, P. polymyxa and B. brevis were entomopathogenic. Larval mortality assessed 7 days post-treatment showed high mortality rates with Btk to lepidopteran larvae (86.6% for P. oleae and 80.9% for P. unionalis) and low mortality against coleopteran pests. B. brevis isolates showed high mortality rates against P. oleae (up to 67.9%). B. licheniformis isolates caused up to 59.2% larval mortality for P. oleae and 43.6% for P. unionalis. Highest coleopteran mortality was achieved by P. polymyxa isolates (up to 55%). According to the 16S rDNA results, isolates of each of the three entomopathogenic strains were similar. Proteins in the strain supernatants were toxic to P. oleae larvae with LC50 values of 10.0 (B. brevis), 12.5 (B. licheniformis) and 37.6 μg/ml (P. polymyxa). Also, P. polymyxa showed an LC50 of 12.4 mg/l against P. scarabaeoides. Our results suggest that entomopathogenic Bacillus present locally in the biodynamic farm could be used in biological control programmes of olive tree pests.
اللغةen
الناشرElsevier Ltd.
الموضوعOrganic farming
Olive tree
Prays oleae
Palpita unionalis
Hylesinus oleiperda
Phloeotribus scarabaeoides
Bacillus sp
Biological control
العنوانIsolation of entomopathogenic Bacillus from a biodynamic olive farm and their pathogenicity to lepidopteran and coleopteran insect pests
النوعArticle
الصفحات72-77
رقم العدد1
رقم المجلد31


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