Studies for determining antimicrobial activity of solenostemma argel (del) hayne. 1-extraction with methanol/water in different proportions

Abstract

Aerial parts of Solenostemma argel plant were successively extracted with methanol/water in different proportions (4 fractions). The phytochemical and chromatographic screening as well as quantitative determination of the flavonoid and saponin contents were carried out to each fraction. The antimicrobial activity of the four fractions against eight bacteria: Staphylococcus aureus; Micrococcus; Streptococcus spp; Bacillus anthracis; E. coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; and Proteus vulgaris and 14 fungi: Fusarium; Aspergillus parasiticus; A. flavus; A. niger; A. candidus; A. glaucus; Penicillum; Chrisosporium; Cr. neoformans; Candida spp; C. albicans; Can. spp 20; Mucor and Rhodotorula were studied. It was clear that the most powerful effect was observed in case of Streptococcus spp.; moderate action against E. coli, B. anthracis; S. aureus; Klebsiella pneumoniae and Proteus vulgaris. The fungicidal activity of fraction No. 1 showed antifungal activity to A. niger; Mucor while fraction 2 showed the activity against A. niger; A. candidus chrisosporium, Cand. albicans, Cand. spp 20 and Rhodotorula. But fraction 3 had an effect on A. niger, Cand. spp 20 and Rhodotorula. On the other hand, fraction 4 was highly effective to A. parasiticus and A. candidus. It was clear that 4 fractions gave different degrees of antifungal activity to the examined 14 fungal species.