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AuthorFois, Alessandro Giuseppe
AuthorPosadino, Anna Maria
AuthorGiordo, Roberta
AuthorCossu, Annalisa
AuthorAgouni, Abdelali
AuthorRizk, Nasser Moustafa
AuthorPirina, Pietro
AuthorCarru, Ciriaco
AuthorZinellu, Angelo
AuthorPintus, Gianfranco
Available date2018-11-26T08:56:32Z
Publication Date2018-10-21
Publication NameOxidative Medicine and Cellular Longevity
Identifierhttp://dx.doi.org/10.1155/2018/2639081
CitationAlessandro Giuseppe Fois, Anna Maria Posadino, Roberta Giordo, et al., “Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients,” Oxidative Medicine and Cellular Longevity, vol. 2018, Article ID 2639081, 8 pages, 2018. https://doi.org/10.1155/2018/2639081
ISSN1942-0900
IdentifierArticle ID 2639081
URIhttp://hdl.handle.net/10576/11182
AbstractIdiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by an exacerbated fibrotic response. Although molecular and cellular determinants involved in the onset and progression of this devastating disease are largely unknown, an aberrant remodeling of the pulmonary vasculature appears to have implications in IPF pathogenesis. Here, we demonstrated for the first time that an increase of reactive oxygen species (ROS) generation induced by sera from IPF patients drives both collagen type I deposition and proliferation of primary human pulmonary artery smooth muscle cells (HPASMCs). IPF sera-induced cellular effects were significantly blunted in cells exposed to the NADPH oxidase inhibitor diphenyleneiodonium (DPI) proving the causative role of ROS and suggesting their potential cellular source. Contrary to IPF naive patients, sera from Pirfenidone-treated IPF patients failed to significantly induce both ROS generation and collagen synthesis in HPASMCs, mechanistically implicating antioxidant properties as the basis for the in vivo effect of this drug.
Languageen
PublisherHindawi Publishing Corporation
SubjectOxidative Stress
Idiopathic pulmonary fibrosis
VSMC
Fibrosis
TitleAntioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients.
TypeArticle
Volume Number2018
ESSN1942-0994
dc.accessType Open Access


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