عرض بسيط للتسجيلة

المؤلفEisa-Beygi, Shahram
المؤلفBenslimane, Fatiha M.
المؤلفEl-Rass, Suzan
المؤلفPrabhudesai, Shubhangi
المؤلفAbdelrasoul, Mahmoud Khatib Ali
المؤلفSimpson, Pippa M.
المؤلفyalcin, Huseyin C.
المؤلفBurrows, Patricia E.
المؤلفRamchandran, Ramani
تاريخ الإتاحة2018-12-19T05:10:51Z
تاريخ النشر2018-09
اسم المنشورArteriosclerosis, Thrombosis, and Vascular Biology
المعرّفhttp://dx.doi.org/10.1161/ATVBAHA.118.311231
الاقتباسEisa-Beygi, S., Benslimane, F. M., El-Rass, S., Prabhudesai, S., Abdelrasoul, M. K. A., Simpson, P. M., ... & Ramchandran, R. (2018). Characterization of endothelial cilia distribution during cerebral-vascular development in zebrafish (Danio rerio). Arteriosclerosis, Thrombosis, and Vascular Biology, 38(12), 2806-2818.
الرقم المعياري الدولي للكتاب1079-5642
معرّف المصادر الموحدhttp://hdl.handle.net/10576/11210
الملخصObjective—Endothelial cells (ECs) sense and respond to flow-induced mechanical stress, in part, via microtubule-based projections called primary cilia. However, many critical steps during vascular morphogenesis occur independent of flow. The involvement of cilia in regulating these stages of cranial vascular morphogenesis is poorly understood because cilia have not been visualized in primary head vessels. The objective of this study was to investigate involvement of cilia in regulating the early stages of cranial vascular morphogenesis. Approach and Results—Using high-resolution imaging of the Tg(kdrl:mCherry-CAAX) y171;(bactin::Arl13b:GFP) zebrafish line, we showed that cilia are enriched in the earliest formed cranial vessels that assemble via vasculogenesis and in angiogenic hindbrain capillaries. Cilia were more prevalent around the boundaries of putative intravascular spaces in primary and angiogenic vessels. Loss of cardiac contractility and blood flow, because of knockdown of cardiac troponin T type 2a (tnnt2a) expression, did not affect the distribution of cilia in primary head vasculature. In later stages of development, cilia were detected in retinal vasculature, areas of high curvature, vessel bifurcation points, and during vessel anastomosis. Loss of genes crucial for cilia biogenesis (ift172 and ift81) induced intracerebral hemorrhages in an EC-autonomous manner. Exposure to high shear stress induced premature cilia disassembly in brain ECs and was associated with intracerebral hemorrhages. Conclusions—Our study suggests a functional role for cilia in brain ECs, which is associated with the emergence and remodeling of the primary cranial vasculature. This cilia function is flow-independent, and cilia in ECs are required for cerebral-vascular stability.
راعي المشروعS. Eisa-Beygi is supported by funds from Kelleigh’s Cause Foundation. R. Ramchandran is also supported by 1R01HL123338,and partly supported by funds from Women’s Health Research Program at the Medical College of Wisconsin. S. Prabhudesai is supported by Department of Pediatrics, and Children’s Research Institute funds to R. Ramchandran. H.C. Yalcin is supported by QNRF (Qatar National Research Fund), National Priority Research Program NPRP 10-0123-170222, and Qatar University internal funds QUUGBRC-2017-3 and QUST-BRC-SPR\2017-1.
اللغةen
الناشرAmerican Heart Association
الموضوعarteriovenous malformations
brain
cilia
endothelial cells
morphogenesis
shear stress
zebrafish
العنوانCharacterization of Endothelial Cilia Distribution During Cerebral-Vascular Development in Zebrafish (Danio rerio)
النوعArticle
الصفحات2806-2818
رقم العدد12
رقم المجلد38
ESSN1524-4636
dc.accessType Abstract Only


الملفات في هذه التسجيلة

الملفاتالحجمالصيغةالعرض

لا توجد ملفات لها صلة بهذه التسجيلة.

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة