Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients
Author | Myronovkij, Severyn |
Author | Negrych, Nazar |
Author | Nehrych, Tetyana |
Author | Tkachenko, Volodymyr |
Author | Souchelnytskyi, Serhiy |
Author | Stoika, Rostyslav |
Author | Kit, Yuriy |
Available date | 2019-02-13T09:55:47Z |
Publication Date | 2016-09-15 |
Publication Name | Protein and Peptide Letters |
Citation | Myronovkij, Severyn. et al. "Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients", Protein and Peptide Letters, Volume 23, Number 9, September 2016, pp. 808-811(4) |
ISSN | 0929-8665 |
Abstract | Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in blood serum of the MS patients. Further examination of TCA-extracted fraction of blood serum of these patients by means of thin-layer chromatography and HPLC gel-filtration allowed detecting 300-500 Da peptides. MALDI TOF/TOF massspectrometry of these peptides showed that they contain Ser-Pro-Cys amino acid sequence. We discussed potential mechanisms of a release of these peptides that were earlier unknown in blood serum of the MS patients. |
Sponsor | NPRP9-453-3-089, QU internal grants |
Language | en |
Publisher | Bentham Science Publishers |
Subject | blood serum Multiple sclerosis peptides Amino Acid Sequence Adult Biomarkers/blood Cysteine/metabolism Female Male Humans Middle Aged Multiple Sclerosis/metabolism Peptides/blood Peptides/chemistry Peptides/isolation & purification Proline/metabolism Serine/metabolism Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Tandem Mass Spectrometry Young Adult |
Type | Article |
Issue Number | 9 |
Volume Number | 23 |
ESSN | 1875-5305 |
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