| Abstract | Breast cancer still remains a major cause of morbidity and mortality among
women in Qatar and worldwide. More recent studies indicate that the diversity and
the composition of the entire set of antigen receptors within tumor-infiltrating
lymphocytes (TILs) is strongly correlated with tumor prognosis and therapeutic
response with breast cancer. Unfortunately, the relationship between somatic
mutational load and TCR diversity of TILs across breast cancer still limited. For
this purpose, first we characterized the somatic mutations of Formalin-Fixed
Paraffin-Embedded breast cancer samples from 79 patients using NGS of a panel
of cancer related genes. Second, we classified and identified the TCRß repertoire
for these 11 samples using the ImmunoSEQ platform. Preliminary data
demonstrated that the 11 patients had high diversity of TCRß-CDR3 within the
tumors. However, there was no statistically significant association between the
somatic mutational loads in the gene panels we sequenced and the number of
productive TCRß-CDR3 rearrangements. |
