Gut Microbiota Dysbiosis in Cafeteria Diet Fed Sprague Dawley Rats
الملخص
Diet plays a major role in the body physiology and metabolism. The quantity,
nature and stability of the macronutrients present in the diet have a major impact on the composition of gut microbiota. Gut microbiota plays a major role in
the body metabolism and leads to obese or lean phenotype. Bacteriodetes, Firmicutes, Proteobacteria and Actinobacteria are the major microbes that inhabit
in the region of the gut. We made an attempt to study the effects of Cafeteria
(CAF) diets and normal chow diets on diet consumption, weight gain, metabolism and composition of gut microbiota in fecal and cecum samples from three
weeks old Sprague Dawley (SD) rats (n = 18/group) using 16S rDNA high
throughput sequencing. Results revealed that distinctive diet based phenotypical
changes were observed in some of the Cafeteria diet fed rats. Interestingly, some
weight gain resistant (WGR) animals in Cafeteria diet fed groups show similar
trend like that of control normal chow fed rats. Fecal microbiome analysis indicates that the ratio of Bacteriodetes is higher than the Firmicutes in cecum samples of Cafeteria diet fed rats whereas no significant difference is found in fecal
samples of Cafeteria diet fed rats and as well as in control rats. Further analysis
of other taxa at the level of family and genus of microbial abundance are also
discussed. Our study suggests that contribution of gut microbiota towards obesity is not at the phylum level, and microbiome composition even at the level of
species or strain may exert impact on the metabolism of the Cafeteria diet.
المجموعات
- العلوم البيولوجية والبيئية [871 items ]
- الأبحاث [96 items ]