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AuthorMukhtar, Imran
AuthorAnwar, Haseeb
AuthorHussain, Ghulam
AuthorRasul, Azhar
AuthorNaqvi, Syed Ali Raza
AuthorFaisal, Muhammad Naeem
AuthorMustafa, Imtiaz
AuthorMalik, Saima
AuthorShaukat, Arslan
AuthorMirza, Osman Asghar
AuthorSohail, Muhammad Umar
Available date2019-09-08T07:44:18Z
Publication Date2019-03-01
Publication NamePakistan journal of pharmaceutical sciences
ISSN1011-601X
URIhttp://hdl.handle.net/10576/11787
AbstractGut microbiome, a new organ; represent targets to alter pharmacokinetics of orally administered drugs. Recently, in vitro trials endorsed the idea that orally administered drugs interact and some of their quantity may be taken up by normal microbiome during transit through gut. Such transport mechanisms in microbiome may compete for drug with the host itself. Currently, no data confirms specific transport system for paracetamol uptake by gut microbiome. In vivo trial was conducted in normal healthy male rats (n=36). Paracetamol was administered orally in a single dose of 75mg/kg to isolate microbial mass after transit of 2, 3, 4, 5 and 6 hours post drug administration. Paracetamol absorbance by microbiome was pursued by injecting extracted microbial lysate in RP-HPLC-UV with C18 column under isocratic conditions at 207nm using acetonitrile and water (25:75 v/v) pH 2.50 as mobile phase. Paracetamol absorbance (14.10±0.75μg/mg of microbial mass) and percent dose recovery (13.16±0.55%) seen at transit of 4 hours was significantly higher (P<0.05) compared to other groups. Study confirms the hypothesis of homology between membrane transporters of the gut microbiome and intestinal epithelium. Orally administered drugs can be absorbed by gut microbes competitively during transit in small intestine and it varies at various transit times.
Languageen
PublisherUniversity of Karachi, Pharmacy College
SubjectMicrobiome
metabolism
Digestion
Pharmacokinetics
TitleDetection of Paracetamol as substrate of the gut microbiome
TypeArticle
Pagination751-757
Issue Number2
Volume Number32
dc.accessType Open Access


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