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المؤلفAugustine, Robin
المؤلفAlhussain, Hashim
المؤلفHasan, Anwarul
المؤلفBadie Ahmed, Mohamed
المؤلفC Yalcin, Huseyin
المؤلفAl Moustafa, Ala-Eddin
تاريخ الإتاحة2019-09-22T04:59:07Z
تاريخ النشر2019-07-01
اسم المنشورBosnian Journal of Basic Medical Science
المعرّفhttp://dx.doi.org/10.17305/bjbms.2019.4372
الاقتباسAugustine R, Alhussain H, Hasan A, Badie Ahmed M, C. Yalcin H, Al Moustafa A-E. A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells. Bosn J of Basic Med Sci [Internet]. 2019Jul.24 [cited 2019Sep.22];00. Available from: https://bjbms.org/ojs/index.php/bjbms/article/view/4372
الرقم المعياري الدولي للكتاب1512-8601
معرّف المصادر الموحدhttp://hdl.handle.net/10576/11897
الملخصCancer metastasis is the leading cause of cancer-related mortality worldwide. To date, several in vitro methodologies have been developed to understand the mechanisms of cancer metastasis and to screen various therapeutic agents against it. Nevertheless, mimicking an in vivo microenvironment in vitro is not possible; while in vivo experiments are complex, expensive and bound with several regulatory requirements. Herein, we report a novel in ovo model that relies on chicken embryo to investigate cancer cell invasion and metastasis to various organs of the body. In this model, we directly inject green fluorescent protein (GFP) expressing cancer cells to the heart of chicken embryo at 3 days of incubation, then monitor cell migration to various organs. To this end, we used a simple tissue processing technique to achieve rapid imaging and quantification of invasive cells. We were able to clearly observe the migration of GFP expressing cancer cells into various organs of chicken embryo. Organ specific variation in cell migration was also observed. Our new slide pressing based tissue processing technique improved the detectability of migrated cells. We herein demonstrate that the use of GFP expressing cancer cells allows easy detection and quantification of migrated cancer cells in the chicken embryo model, which minimizes the time and effort required in this types of studies compared to conventional histopathological analysis. In conclusion, our investigation provides a new cancer metastasis model that can be further improved to include more complex aspects, such as the use of multiple cell lines and anti-metastatic agents, thus opening new horizons in cancer biology and pharmaceutical research.
اللغةen
الناشرAssociation of Basic Medical Sciences of Federation of Bosnia and Herzegovina
الموضوعTumor invasion
in ovo models
chicken embryo model
metastasis
GFP cells
العنوانA novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells.
النوعArticle
ESSN1840-4812
dc.accessType Open Access


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