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المؤلفYadav P.
المؤلفMaharjan J.
المؤلفKorpole S.
المؤلفPrasad G.S.
المؤلفSahni G.
المؤلفBhattarai T.
المؤلفSreerama L.
تاريخ الإتاحة2019-10-06T09:38:34Z
تاريخ النشر2018
اسم المنشورFrontiers in Bioengineering and Biotechnology
المصدرScopus
الرقم المعياري الدولي للكتاب2296-4185
معرّف المصادر الموحدhttp://dx.doi.org/10.3389/fbioe.2018.00065
معرّف المصادر الموحدhttp://hdl.handle.net/10576/12070
الملخصAnoxybacillus kamchatkensis NASTPD13 used herein as a source for thermostable alkaline xylanase were isolated from Paudwar Hot Springs, Nepal. NASTPD13 cultured at 60°C, pH 7 and in presence of inorganic (ammonium sulfate) or organic (yeast extract) nitrogen sources, produced maximum xylanase enzyme. Xylanase production in the cultures was monitored by following the ability of culture media to hydrolyze beech wood xylan producing xylooligosaccharide and xylose by thin layer chromatography (TLC). The extracellular xylanase was isolated from optimized A. kamchatkensis NASTPD13 cultures by ammonium sulfate (80%) precipitation; the enriched xylanase preparation was dialyzed and purified using Sephadex G100 column chromatography. The purified xylanaseshowed 11-fold enrichment with a specific activity of 33 U/mg and molecular weight were37 kDa based on SDS-PAGE and PAGE-Zymography. The optimum pH and temperature of purified xylanase was 9.0 and 65°C respectively retainingmore than 50% of its maximal activity over a broad range of pH (6–9) and temperature (30–65°C). With beech wood xylan, the enzyme showed Km 0.7 mg/ml and Vmax 66.64 μM/min/mg The xylanase described herein is a secretory enzyme produced in large quantities by NASTPD13 and is a novel thermostable, alkaline xylanase with potential biotechnological applications.
اللغةen
الناشرFrontiers Media S.A.
الموضوعAlkaline
Anoxybacillus
Hot springintroduction
Thermostable
Xylanase
العنوانProduction, purification, and characterization of thermostable alkaline xylanase from Anoxybacillus kamchatkensis NASTPD13
النوعArticle
رقم العددMAY
رقم المجلد6
dc.accessType Open Access


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