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AuthorAl Soubaihi R.M.
AuthorFuresi G.
AuthorSaoud K.M.
AuthorAl-Muhtaseb S.A.
AuthorKhatat A.E.
AuthorDelogu L.G.
AuthorDutta J.
Available date2019-11-03T11:47:37Z
Publication Date2018
Publication NameColloids and Surfaces B: Biointerfaces
ResourceScopus
ISSN9277765
URIhttp://dx.doi.org/10.1016/j.colsurfb.2018.09.022
URIhttp://hdl.handle.net/10576/12206
AbstractSilica nanosheets (SiO 2 NS) are considered to be a promising material in clinical practice for diagnosis and therapy applications. However, an appropriate surface functionalization is essential to guarantee high biocompatibility and molecule loading ability. Although SiO 2 NS are chemically stable, its effects on immune systems are still being explored. In this work, we successfully synthesized a novel 2D multilayer SiO 2 NS and SiO 2 NS coated with carbon (C/SiO 2 NS), and evaluated their impact on human Peripheral Blood Mononuclear Cells (PBMCs) and some immune cell subpopulations. We demonstrated that the immune response is strongly dependent on the surface functionalities of the SiO 2 NS. Ex vivo experiments showed an increase in biocompatibility of C/SiO 2 NS compared to SiO 2 NS, resulting in a lowering of hemoglobin release together with a reduction in cellular toxicity and cellular activation. However, none of them are directly involved in the activation of the acute inflammation process with a consequent release of pro-inflammatory cytokines. The obtained results provide an important direction towards the biomedical applications of silica nanosheets, rendering them an attractive material for the development of future immunological therapies.
SponsorThis publication was made possible by an NPRP award [NPRP 6- 892-1-169 ] from the Qatar National Research Fund (a member of The Qatar Foundation) . The biocompatibility studies were partially supported through FLAG-ERA MIUR (G-IMMUNOMICS project) , the European Union HORIZON 2020 research and innovation program under MSCA RISE 2016 project Carbo-Immap grant. n. 734381 and Italian MIUR (project: 2015TWP83Z ). The statements made herein are solely the responsibility of the authors.
Languageen
PublisherElsevier B.V.
Subject2D materials
Apoptosis assay
Bio compatibility
Carbon coated SiO 2
Cell toxicity
Cell viability
Characterization
Hemolysis tests
Immune cell
Layered silica
Nano-sheets
Secretion assay
Silica
Synthesis
Tumor necrosis factor alpha (TNF?)
TitleSilica and carbon decorated silica nanosheet impact on primary human immune cells
TypeArticle
Pagination779 - 789
Volume Number172
dc.accessType Abstract Only


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