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المؤلفAlasmar, Reem Moath
المؤلفVaradharajan, Kavitha
المؤلفShanmugakonar, Muralitharan
المؤلفAl-Naemi, Hamda A.
تاريخ الإتاحة2019-12-12T07:34:46Z
تاريخ النشر2019-09-15
اسم المنشورFood and Nutrition Sciences
المعرّفhttp://dx.doi.org/10.4236/aim.2019.99047
الاقتباسAlasmar, R.M., Varadharajan, K., Shanmugakonar, M. and Al-Naemi, H.A. (2019) Gut Microbiota Dysbiosis and Their Impact on Biochemical and Inflammatory Profiles in Cafeteria Diet Fed Sprague Dawley Rats. Advances in Microbiology, 9, 775-789. https://doi.org/10.4236/aim.2019.99047
الرقم المعياري الدولي للكتاب2157-944X
معرّف المصادر الموحدhttp://hdl.handle.net/10576/12386
الملخصGut microbiota plays a major role in regulating the host metabolism and immune system. However, the structure of microbiome population is altered constantly by diverse factors including diet and environment. In particular, the gut microbiome dynamics is influenced by diet composition and their associated metabolites. Many studies in the recent past reported on diet induced dysbiosis in the gut microbiome, the modulating ratio between Firmicutes and Bacteroidetes plays a central role in maintaining the microbiome diversity in the gut and their abundance regulates obese conditions. Although there are several reports on gut microbial dysbiosis (Firmicutes/Bacteroidetes) at phylum level but only few of them highlight at the genera level. In the present study, we focused more on the impact of cafeteria diet (CAF) with respect to the gut microbiome richness at the genera level in SD rats. Three weeks old Sprague Dawley (SD) rats were fed with normal chow diet and cafeteria diet (CAF). After 10 weeks, serum, tissue samples (small intestine and cecum), cecum fecal and fecal pellet were collected. Biochemical analysis from serum, Gene expression analysis of pro-inflammatory markers from tissues and microbiome analysis from fecal samples were analysed. CAF diet fed rat in the present study developed obesity with increased body weight, few of them developed the resistance to weight gain (WGR) and these animal shows significantly increased abundance of Bacteriodetes-Prevotella compared to obese animals. This study suggests that detailed research needed to address the contribution of microbiota abundance at the genera level. We further explored the influence of diet induced microbiota changes on immune response; increase in Bacteroidetes leads to increased LPS and this in turn elicit immune reaction in the gut. Moreover, variation in gut microbiota also affects the gut barrier function (Tight junction proteins) and increase metabolic endotoxemia that leads to activation of innate immune system and low-grade inflammation. Further studies on these lines targeting the microbiota changes in the gut at the genera level is warranted to gain more knowledge.
اللغةen
الناشرScientific Research Publishing
الموضوعGut Microbiota
Cafeteria Diet
Obesity
Inflammatory Markers
RT-PCR
العنوانGut Microbiota Dysbiosis and Their Impact on Biochemical and Inflammatory Profiles in Cafeteria Diet Fed Sprague Dawley Rats
النوعArticle
الصفحات775-789
رقم العدد9
رقم المجلد9
ESSN2157-9458
dc.accessType Open Access


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