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AuthorFilip, Jaroslav
AuthorZavahir, Sifani
AuthorKlukova, Ludmila
AuthorTkac, Jan
AuthorKasak, Peter
Available date2020-09-20T08:35:36Z
Publication Date2017
Publication NameJournal of Electroanalytical Chemistry
ResourceScopus
ISSN15726657
URIhttp://dx.doi.org/10.1016/j.jelechem.2017.04.019
URIhttp://hdl.handle.net/10576/16159
AbstractLectins, which are proteins with selective affinity to glycans or glycoproteins, have been recognized as promising agents for the construction of devices for the detection of specific glycoproteins and for glycoprofiling. This allows for the exploration of new potential biomarkers and for early diagnosis by detection of already known glycosylated biomarkers. In this work, immobilization of Concanavalin A (ConA) lectin on an electrochemically reduced graphene oxide (ErGO)/thionine (Thi) surface via glutaraldehyde (GA) crosslinking is investigated and applied for the impedimetric detection of the glycoprotein invertase (INV). An attachment of ConA/GA aggregates to the ErGO/Thi surface leads to a biosensor with a linear response in the concentration range of 10 14-10 8 mol for INV and a sensitivity of 6.1% of RCT change per decade of INV concentration. The sensitivity towards a negative control, i.e., INV with oxidized glycan moieties, is 2.97-fold lower than that towards INV. These findings provide a platform for the development of lectin-based, miniature and cheap biosensors for possible future disease diagnosis.
SponsorThis work was made possible by NPRP grant no. NPRP-6-381-1-078 from the Qatar National Research Fund (a member of Qatar Foundation).
Languageen
PublisherElsevier B.V.
SubjectAffinity biosensor
Concanavalin A
Invertase
Reduced graphene oxide
Thionine
TitleImmobilization of concanavalin A lectin on a reduced graphene oxide-thionine surface by glutaraldehyde crosslinking for the construction of an impedimetric biosensor
TypeArticle
Pagination156-163
Volume Number794
dc.accessType Abstract Only


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