Show simple item record

AuthorOumayma, Kahla
AuthorMelliti Ben Garali, Sondes
AuthorKarray, Fatma
AuthorBen Abdallah, Manel
AuthorKallel, Najwa
AuthorMhiri, Najla
AuthorZaghden, Hatem
AuthorBarhoumi, Badreddine
AuthorPringault, Olivier
AuthorQuéméneur, Marianne
AuthorTedetti, Marc
AuthorSayadi, Sami
AuthorSakka Hlaili, Asma
Available date2020-09-23T04:59:17Z
Publication Date2021-01-10
Publication NameScience of The Total Environment
Identifierhttp://dx.doi.org/10.1016/j.scitotenv.2020.141399
CitationKahla, O., Garali, S. M. B., Karray, F., Abdallah, M. B., Kallel, N., Mhiri, N., ... & Tedetti, M. Efficiency of benthic diatom-associated bacteria in the removal of benzo (a) pyrene and fluoranthene. Science of the Total Environment, 751, 141399.
ISSN00489697
URIhttps://www.sciencedirect.com/science/article/pii/S0048969720349287
URIhttp://hdl.handle.net/10576/16247
AbstractWe investigated the efficiency of a benthic diatom-associated bacteria in removing benzo(a)pyrene (BaP) and fluoranthene (Flt). The diatom, isolated from a PAH-contaminated sediment of the Bizerte Lagoon (Tunisia), was exposed in axenic and non-axenic cultures to PAHs over 7 days. The diversity of the associated bacteria, both attached (AB) and free-living bacteria (FB), was analyzed by the 16S rRNA amplicon sequencing. The diatom, which maintained continuous growth under PAH treatments, was able to accumulate BaP and Flt, with different efficiencies between axenic and non-axenic cultures. Biodegradation, which constituted the main process for PAH elimination, was enhanced in the presence of bacteria, indicating the co-metabolic synergy of microalgae and associated bacteria in removing BaP and Flt. Diatom and bacteria showed different capacities in the degradation of BaP and Flt. Nitzschia sp. harbored bacterial communities with a distinct composition between attached and free-living bacteria. The AB fraction exhibited higher diversity and abundance relative to FB, while the FB fraction contained genera with the known ability of PAH degradation, such as Marivita, Erythrobacter, and Alcaligenes. Moreover, strains of Staphylococcus and Micrococcus, isolated from the FB community, showed the capacity to grow in the presence of crude oil. These results suggest that a “benthic Nitzschia sp.-associated hydrocarbon-degrading bacteria” consortium can be applied in the bioremediation of PAH-contaminated sites.
SponsorThis work was supported by IRD through the Laboratoire Mixte International (LMI) COSYS-Med (Contaminants et Ecosystèmes Sud Méditerranéens). English grammar and syntax of the manuscript were revised by Proof-Reading-Service.com. We thank anonymous reviewers for useful suggestions.
Languageen
PublisherElsevier
SubjectPAH accumulation
PAH biodegradation
Bizerte Lagoon
Nitzschia sp. phycosphere
Hydrocarbon-degrading bacteria
TitleEfficiency of benthic diatom-associated bacteria in the removal of benzo(a)pyrene and fluoranthene
TypeArticle
Pagination141399
Volume Number751
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record