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AuthorLópez-Ozuna, Vanessa M
AuthorGupta, Ishita
AuthorKiow, Ryan Liu Chen
AuthorMatanes, Emad
AuthorKheraldine, Hadeel
AuthorYasmeen, Amber
AuthorKhalil, Ashraf
AuthorVranic, Semir
AuthorAl Moustafa, Ala-Eddin
AuthorFarsi, Halema F Al
Available date2020-09-27T05:02:38Z
Publication Date2020-09-18
Publication NameToxics
Identifierhttp://dx.doi.org/10.3390/toxics8030073
CitationLópez-Ozuna, V.M.; Gupta, I.; Kiow, R.L.C.; Matanes, E.; Kheraldine, H.; Yasmeen, A.; Khalil, A.; Vranic, S.; Al Moustafa, A.-E.; Farsi, H.F.A. Water-Pipe Smoking Exposure Deregulates a Set of Genes Associated with Human Head and Neck Cancer Development and Prognosis. Toxics 2020, 8, 73.
URIhttp://hdl.handle.net/10576/16308
AbstractWater-pipe smoking (WPS) is becoming the most popular form of tobacco use among the youth, especially in the Middle East, replacing cigarettes rapidly and becoming a major risk of tobacco addiction worldwide. Smoke from WPS contains similar toxins as those present in cigarette smoke and is linked directly with different types of cancers including lung and head and neck (HN) carcinomas. However, the underlying molecular pathways and/or target genes responsible for the carcinogenic process are still unknown. In this study, human normal oral epithelial (HNOE) cells, NanoString PanCancer Pathways panel of 770 gene transcripts and quantitative real-time polymerase chain reaction (qRT-PCR) analysis were applied to discover differentially expressed genes (DEG) modulated by WPS. In silico analysis was performed to analyze the impact of these genes in HN cancer patient's biology and outcome. We found that WPS can induce the epithelial-mesenchymal transition (EMT: hallmark of cancer progression) of HNOE cells. More significantly, our analysis of NanoString revealed 23 genes deregulated under the effect of WPS, responsible for the modulation of cell cycle, proliferation, migration/invasion, apoptosis, signal transduction, and inflammatory response. Further analysis was performed using qRT-PCR of HNOE WPS-exposed and unexposed cells supported the reliability of our NanoString data. Moreover, we demonstrate those DEG to be upregulated in cancer compared with normal tissue. Using the Kaplan-Meier analysis, we observed a significant association between WPS-deregulated genes and relapse-free survival/overall survival in HN cancer patients. Our findings imply that WPS can modulate EMT as well as a set of genes that are directly involved in human HN carcinogenesis, thereby affecting HN cancer patients' survival.
SponsorQatar University, grant numbers: QUCP-CMED-2019-1, QUCG-CMED-20/21-2 & QUHI-CMED-19/20-1.
Languageen
PublisherMDPI
Subjectgene dysregulation
head and neck cancers
oral epithelial cells
smoke
water pipe
TitleWater-Pipe Smoking Exposure Deregulates a Set of Genes Associated with Human Head and Neck Cancer Development and Prognosis.
TypeArticle
Issue Number3
Volume Number8
ESSN2305-6304
dc.accessType Open Access


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