Application of a flow-induced stress wave and investigation of associated injuries on cell monolayers using a parallel plate flow chamber
Author | Shurbaji, Samar |
Author | Al-Ruweidi, Mahmoud Khatib A.A. |
Author | Ali, Fatma Hassan |
Author | Benslimane, Fatiha M. |
Author | Yalcin, Huseyin C. |
Available date | 2020-10-15T05:12:59Z |
Publication Date | 2020 |
Publication Name | Methods and Protocols |
Identifier | http://dx.doi.org/10.3390/mps3040065 |
Citation | Shurbaji S, Al-Ruweidi MKAA, Ali FH, Benslimane FM, Yalcin HC. Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber. Methods Protoc. 2020;3(4):E65. Published 2020 Sep 24. doi:10.3390/mps3040065 |
Abstract | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells. |
Language | en |
Publisher | MDPI |
Subject | Bubble Cell injuries Endothelial cells Epithelial cells Flow Hemodynamics Parallel plate flow chamber Stress Viability |
Type | Article |
Issue Number | 4 |
Volume Number | 3 |
ESSN | 2409-9279 |
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