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AuthorShurbaji, Samar
AuthorAl-Ruweidi, Mahmoud Khatib A.A.
AuthorAli, Fatma Hassan
AuthorBenslimane, Fatiha M.
AuthorYalcin, Huseyin C.
Available date2020-10-15T05:12:59Z
Publication Date2020
Publication NameMethods and Protocols
Identifierhttp://dx.doi.org/10.3390/mps3040065
CitationShurbaji S, Al-Ruweidi MKAA, Ali FH, Benslimane FM, Yalcin HC. Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber. Methods Protoc. 2020;3(4):E65. Published 2020 Sep 24. doi:10.3390/mps3040065
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85091489157&origin=inward
URIhttp://hdl.handle.net/10576/16451
Abstract© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells.
Languageen
PublisherMDPI
SubjectBubble
Cell injuries
Endothelial cells
Epithelial cells
Flow
Hemodynamics
Parallel plate flow chamber
Stress
Viability
TitleApplication of a flow-induced stress wave and investigation of associated injuries on cell monolayers using a parallel plate flow chamber
TypeArticle
Issue Number4
Volume Number3
ESSN2409-9279
dc.accessType Open Access


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