Quantification of bioburden in LARC'S Vivarium

Abstract

Sanitization of cleanrooms in animal facilities is important as it ensures the level of contamination by different means including personnel activities and failures of HVAC system.Cleanrooms are highly controlled to provide a protective working environment . Designed to minimize the transfer of contamination .In our monitoring program we assess the total microbial load and molecules present in living cells as an energy source ATP (adenosine triphosphate). Data of RODAC plate count and ATPase assay (adenosine triphosphate) method from one year of environmental monitoring are discussed