Immobilization of bilirubin oxidase on graphene oxide flakes with different negative charge density for oxygen reduction. The effect of GO charge density on enzyme coverage, electron transfer rate and current density
المؤلف | Filip, Jaroslav |
المؤلف | Andicsova-Eckstein, Anita |
المؤلف | Vikartovska, Alica |
المؤلف | Tkac, Jan |
تاريخ الإتاحة | 2020-11-26T11:21:09Z |
تاريخ النشر | 2017 |
اسم المنشور | Biosensors and Bioelectronics |
المصدر | Scopus |
الملخص | Previously we showed that an effective bilirubin oxidase (BOD)-based biocathode using graphene oxide (GO) could be prepared in 2 steps: 1. electrostatic adsorption of BOD on GO; 2. electrochemical reduction of the BOD-GO composite to form a BOD-ErGO (electrochemically reduced GO) film on the electrode. In order to identify an optimal charge density of GO for BOD-ErGO composite preparation, several GO fractions differing in an average flake size and ζ-potential were prepared using centrifugation and consequently employed for BOD-ErGO biocathode preparation. A simple way to express surface charge density of these particular GO nanosheets was developed. The values obtained were then correlated with biocatalytic and electrochemical parameters of the prepared biocathodes, i.e. electrocatalytically active BOD surface coverage (Γ), heterogeneous electron transfer rate (kS) and a maximum biocatalytic current density. The highest bioelectrocatalytic current density of (597±25) μA cm−2 and the highest Γ of (23.6±0.9) pmol cm−2 were obtained on BOD-GO composite having the same moderate negative charge density, but the highest kS of (79.4±4.6) s−1 was observed on BOD-GO composite having different negative charge density. This study is a solid foundation for others to consider the influence of a charge density of GO on direct bioelectrochemistry/bioelectrocatalysis of other redox enzymes applicable for construction of biosensors, bioanodes, biocathodes or biofuel cells. |
راعي المشروع | The financial support received from VEGA 2/0162/14 and VEGA 2/0090/16 ; and from APVV 0282-11 is acknowledged. This work was funded by Centre of Excellence for Glycomics , supported by the Research & Development Operational Program funded by the ERDF ( ITMS 26240120031 ). This publication is the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions ? Stage I, ITMS No.: 26240120007 supported by the Research & Development Operational Program funded by the ERDF. |
اللغة | en |
الناشر | Elsevier Ltd |
الموضوع | Bilirubin oxidase Biocathode Direct bioelectrocatalysis Graphene oxide Oxygen reduction |
النوع | Article |
الصفحات | 384-389 |
رقم المجلد | 89 |
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