Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals
المؤلف | Younes, Salma |
المؤلف | Al-Jighefee, Hadeel |
المؤلف | Shurrab, Farah |
المؤلف | Al-Sadeq, Duaa W. |
المؤلف | Younes, Nadin |
المؤلف | Dargham, Soha R. |
المؤلف | Al-Dewik, Nader |
المؤلف | Qotba, Hamda |
المؤلف | Syed, Mohamed |
المؤلف | Alnuaimi, Ahmed |
المؤلف | Yassine, Hadi M. |
المؤلف | Tang, Patrick |
المؤلف | Abu-Raddad, Laith J. |
المؤلف | Nasrallah, Gheyath K. |
تاريخ الإتاحة | 2021-01-27T10:59:44Z |
تاريخ النشر | 2020-01-25 |
اسم المنشور | Microorganisms |
المعرّف | http://dx.doi.org/10.3390/microorganisms9020245 |
الاقتباس | Younes, S.; Al-Jighefee, H.; Shurrab, F.; Al-Sadeq, D.W.; Younes, N.; Dargham, S.R.; Al-Dewik, N.; Qotba, H.; Syed, M.; Alnuaimi, A.; et al. Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals. Microorganisms 2021, 9, 245. https:// doi.org/10.3390/microorganisms 9020245 |
الملخص | Abstract: To support the deployment of serology assays for population screening during the COVID-19 pandemic, we compared the performance of three fully automated SARS-CoV-2 IgG assays: Mindray CL-900i® (target: spike [S] and nucleocapsid [N]), BioMérieux VIDAS®3 (target: receptor-binding domain [RBD]) and Diasorin LIAISON®XL (target: S1 and S2 subunits). A total of 111 SARS-CoV-2 RT-PCR- positive samples collected at ≥ 21 days post symptom onset, and 127 prepandemic control samples were included. Diagnostic performance was assessed in correlation to RT-PCR and a surrogate virus-neutralizing test (sVNT). Moreover, cross-reactivity with other viral antibodies was investigated. Compared to RT-PCR, LIAISON®XL showed the highest overall specificity (100%), followed by VIDAS®3 (98.4%) and CL-900i® (95.3%). The highest sensitivity was demonstrated by CL-900i® (90.1%), followed by VIDAS®3 (88.3%) and LIAISON®XL (85.6%). The sensitivity of all assays was higher in symptomatic patients (91.1–98.2%) compared to asymptomatic patients (78.4–80.4%). In correlation to sVNT, all assays showed excellent sensitivities (92.2–96.1%). In addition, VIDAS®3 demonstrated the best correlation (r = 0.75) with the sVNT. The present study provides insights on the performance of three fully automated assays, which could help diagnostic laboratories in the choice of a particular assay according to the intended use |
اللغة | en |
الناشر | MDPI |
الموضوع | COVID-19 SARS-CoV-2 serology sensitivity specificity neutralizing antibodies surrogate virus neutralization test (sVNT) |
النوع | Article |
رقم المجلد | 9 |
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