Distinct antibody repertoires against endemic human coronaviruses in children and adults.
Author | Khan, Taushif |
Author | Rahman, Mahbuba |
Author | Al Ali, Fatima |
Author | Huang, Susie Sy |
Author | Ata, Manar |
Author | Zhang, Qian |
Author | Bastard, Paul |
Author | Liu, Zhiyong |
Author | Jouanguy, Emmanuelle |
Author | Beziat, Vivien |
Author | Cobat, Aurélie |
Author | Nasrallah, Gheyath K |
Author | Yassine, Hadi M |
Author | Smatti, Maria K |
Author | Saeed, Amira |
Author | Vandernoot, Isabelle |
Author | Goffard, Jean-Christophe |
Author | Smits, Guillaume |
Author | Migeotte, Isabelle |
Author | Haerynck, Filomeen |
Author | Meyts, Isabelle |
Author | Abel, Laurent |
Author | Casanova, Jean-Laurent |
Author | Hasan, Mohammad R |
Author | Marr, Nico |
Available date | 2021-02-23T06:30:55Z |
Publication Date | 2021-01-01 |
Publication Name | JCI Insight |
Identifier | http://dx.doi.org/10.1172/jci.insight.144499 |
Citation | Khan, T., Rahman, M., Al Ali, F., Huang, S. S., Ata, M., Zhang, Q., ... & Marr, N. (2020). Distinct antibody repertoires against endemic human coronaviruses in children and adults. bioRxiv. |
Abstract | Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these "common cold" viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires in 231 children and 1168 adults using phage-immunoprecipitation sequencing. Seroprevalence of antibodies to endemic HCoVs ranged between ~4 and 27% depending on the species and cohort. We identified at least 136 novel linear B cell epitopes. Antibody repertoires against endemic HCoVs were qualitatively different between children and adults in that anti-HCoV IgG specificities more frequently found among children targeted functionally important and structurally conserved regions of the spike, nucleocapsid and matrix proteins. Moreover, antibody specificities targeting the highly conserved fusion peptide region and S2' cleavage site of the spike protein were broadly cross-reactive with peptides of epidemic human and non-human coronaviruses. In contrast, an acidic tandem repeat in the N-terminal region of the Nsp3 subdomain of the HCoV-HKU1 polyprotein was the predominant target of antibody responses in adult donors. Our findings shed light on the dominant species-specific and pan-CoV target sites of human antibody responses to coronavirus infection, thereby providing important insights for the development of prophylactic or therapeutic monoclonal antibodies and vaccine design. |
Sponsor | This work was supported in part by a grant from the Qatar National Research Fund (PPM1-1220-150017) and funds from Sidra Medicine. I Meyts is a Senior Clinical Investigator at the Research Foundation — Flanders and is supported by the CSL Behring Chair of Primary Immunodeficiencies; by the KU Leuven C1 grant C16/18/007; by a VIB GC PID grant; by FWO grants G0C8517N, G0B5120N, and G0E8420N; and by the Jeffrey Modell Foundation. The ULB Center of Human Genetics is supported by the Fonds Erasme. |
Language | en |
Publisher | American Society for Clinical Investigation |
Subject | Immunoglobulins Immunology Infectious disease |
Type | Article |
Issue Number | 4 |
Volume Number | 6 |
ESSN | 2379-3708 |
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