Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells
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Date
2021-06-30Author
Mohamed Qasim, Al-FahdawiAl-Doghachi, Faris A.J.
Abdullah, Qasim Khlaif
Hammad, Ruaa Tareq
Rasedee, Abdullah
Ibrahim, Wisam Nabeel
Alshwyeh, Hussah Abdullah
Alosaimi, Areej A
Aldosary, Sahar Khamees
Eid, Eltayeb E.M.
Rosli, Rozita
Taufiq-Yap, Y.H.
Al-Haj, Nagi A.
Al-Qubaisi, Mothanna Sadiq
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The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound.
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