Oxidative stress cytotoxicity induced by platinum-doped magnesia nanoparticles in cancer cells
Author | Mohamed Qasim, Al-Fahdawi |
Author | Al-Doghachi, Faris A.J. |
Author | Abdullah, Qasim Khlaif |
Author | Hammad, Ruaa Tareq |
Author | Rasedee, Abdullah |
Author | Ibrahim, Wisam Nabeel |
Author | Alshwyeh, Hussah Abdullah |
Author | Alosaimi, Areej A |
Author | Aldosary, Sahar Khamees |
Author | Eid, Eltayeb E.M. |
Author | Rosli, Rozita |
Author | Taufiq-Yap, Y.H. |
Author | Al-Haj, Nagi A. |
Author | Al-Qubaisi, Mothanna Sadiq |
Available date | 2021-03-21T04:48:44Z |
Publication Date | 2021-06-30 |
Publication Name | Biomedicine & Pharmacotherapy |
Identifier | http://dx.doi.org/10.1016/j.biopha.2021.111483 |
ISSN | 07533322 |
Abstract | The aim of this study was to prepare, characterize, and determine the in vitro anticancer effects of platinum-doped magnesia (Pt/MgO) nanoparticles. The chemical compositions, functional groups, and size of nanoparticles were determined using X-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy, and scanning electron microscopy. Pt/MgO nanoparticles were cuboid and in the nanosize range of 30–50 nm. The cytotoxicity of Pt/MgO nanoparticles was determined via the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide assay on the human lung and colonic cancer cells (A549 and HT29 respectively) and normal human lung and colonic fibroblasts cells (MRC-5 and CCD-18Co repectively). The Pt/MgO nanoparticles were relatively innocuous to normal cells. Pt/MgO nanoparticles downregulated Bcl-2 and upregulated Bax and p53 tumor suppressor proteins in the cancer cells. Pt/MgO nanoparticles also induced production of reactive oxygen species, decreased cellular glutathione level, and increased lipid peroxidation. Thus, the anticancer effects of Pt/MgO nanoparticles were attributed to the induction of oxidative stress and apoptosis. The study showed the potential of Pt/MgO nanoparticles as an anti-cancer compound. |
Language | en |
Publisher | Elsevier |
Subject | Pt/MgO nanoparticles Cytotoxicity Oxidative stress Lipid peroxidation Apoptosis Lung cancer Colonic cancer |
Type | Article |
Volume Number | 138 |
Open Access user License | http://creativecommons.org/licenses/by/4.0/ |
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