Prevalence and Phylogenetic Analysis of Parvovirus (B19V) among Blood Donors with Different Nationalities Residing in Qatar
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عرض / فتح
التاريخ
2021-03-25المؤلف
Abdelrahman, DouaAl-Sadeq, Duaa W.
Smatti, Maria K.
Taleb, Sara A.
AbuOdeh, Raed O.
Al-Absi, Enas S.
Al-Thani, Asmaa A.
Coyle, Peter. V.
Al-Dewik, Nader
Al Qahtani, Ahmed A.
Yassine, Hadi M.
Nasrallah, Gheyath K.
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البيانات الوصفية
عرض كامل للتسجيلةالملخص
Human parvovirus (B19V) is the causative agent of erythema infectiosum in children and
is linked to a wide range of clinical manifestations. Studies related to B19V prevalence in the Middle
East and North Africa (MENA) region and other parts of Asia are very scarce. The objectives of
this study were to estimate the seroprevalence (anti-B19V IgM and IgG), the viremia rate (B19V
DNA), and the circulating genotypes of B19V among blood donors in Qatar. Methods: Donors’
blood samples (n = 5026) from different nationalities, mainly from the MENA region and South
East Asia, were collected from 2014–2016. Samples were tested for the B19V DNA using RT-PCR.
Furthermore, 1000 selected samples were tested to determine the seroprevalence of B19V antibodies
using enzyme-linked immunosorbent assay (ELISA). Genotyping was performed on 65 DNA positive
samples by sequencing of nested PCR fragments (NS1-VP1u region, 927 nt). Results: Only 1.4%
(70/5026) of the samples had detectible B19V DNA in their blood. B19V DNA prevalence statistically
decreased with age (p = 0.03). Anti-B19V IgG was detected in 60.3% (561/930) of the tested samples,
while only 2.1% (20/930) were IgM-positive and 1.2% (11/930) were both IgM- and IgG-positive.
B19V genotyping showed a predominance of Genotype 1 (100%). Sequence analysis of the NS1-VP1u
region revealed 139 mutation sites, some of which were amino acid substitutions. Conclusion: Our
results indicated a relatively high seroprevalence of B19V in Qatar. Most importantly, B19 DNA was
detected among Qatari and non-Qatari blood donors. Therefore, blood banks in Qatar might need
to consider screening for B19V, especially when transfusion is intended for high-risk populations,
including immunocompromised patients.
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- الأبحاث [709 items ]
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