Effects of electron acceptors on removal of antibiotic resistant Escherichia coli, resistance genes and class 1 integrons under anaerobic conditions
Author | Yuan, Heyang |
Author | Miller, Jennifer H. |
Author | Abu-Reesh, Ibrahim M. |
Author | Pruden, Amy |
Author | He, Zhen |
Available date | 2021-04-22T13:00:29Z |
Publication Date | 2016 |
Publication Name | Science of the Total Environment |
Resource | Scopus |
Abstract | naerobic biotechnologies can effectively remove antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), but there is a need to better understand the mechanisms. Here we employ bioelectrochemical systems (BES) as a platform to investigate the fate of a native tetracycline and sulfonamide-resistant Escherichia coli strain and its ARGs. The E. coli strain carrying intI1, sulI and tet(E) was isolated from domestic wastewater and dosed into a tubular BES. The BES was first operated as a microbial fuel cell (MFC), with aeration in the cathode, which resulted in enhanced removal of E. coli and ARGs by ~ 2 log (i.e., order of magnitude) when switched from high current to open circuit operation mode. The BES was then operated as a microbial electrolysis cell (MEC) to exclude the effects of oxygen diffusion, and the removal of E. coli and ARGs during the open circuit configuration was again 1–2 log higher than that at high current mode. Significant correlations of E. coli vs. current (R2 = 0.73) and ARGs vs. E. coli (R2 ranged from 0.54 to 0.87), and the fact that the BES substrate contained no electron acceptors, implied that the persistence of the E. coli and its ARGs was determined by the availability of indigenous electron acceptors in the BES, i.e., the anode electrode or the electron shuttles generated by the exoelectrogens. Subsequent experiments with pure-culture tetracycline and sulfonamide-resistant E. coli being incubated in a two-chamber MEC and serum bottles demonstrated that the E. coli could survive by respiring anode electrode and/or electron shuttles released by exoelectrogens, and ARGs persisted with their host E. coli. |
Sponsor | The authors thank Dr. Husen Zhang at Leidos Biomedical Research, Inc. for the assistance with qPCR. This work was made possible by NPRP grant # 6-289-2-125 from the Qatar National Research Fund (a member of Qatar Foundation). The statements made herein are solely the responsibility of the authors. |
Language | en |
Publisher | Elsevier B.V. |
Subject | Anodes Antibiotics Bacteria Electrodes Electrons Escherichia coli Fuel cells Genes Reconfigurable hardware Regenerative fuel cells Sulfur compounds Anaerobic conditions Antibiotic resistance genes Antibiotic-resistant bacteria Antibiotic-resistant escherichia coli Antibiotic-resistant genes Bio-electrochemical systems Bioelectrochemical systems (BES) Electron acceptor Microbial fuel cells electron acceptor oxidizing agent sulfonamide tetracycline unclassified drug antiinfective agent sulfonamide tetracycline derivative anoxic conditions antibiotic resistance biotechnology electrochemistry electron fecal coliform wastewater wastewater treatment aeration anaerobic reactor anode antibiotic resistance Article bacterial gene bacterial survival bacterium culture bacterium isolation bioelectrochemical system cathode controlled study correlation analysis electric current electrical equipment electrode Escherichia coli exoelectrogen integron intI1 gene microbial electrolysis cell microbial fuel cell nonhuman oxygen diffusion priority journal proton transport sulI gene tet gene waste water waste water management anaerobic growth electron Escherichia coli genetics integron microbiology physiology sewage tetracycline resistance Escherichia coli Anaerobiosis Anti-Bacterial Agents Drug Resistance, Bacterial Electrons Escherichia coli Genes, Bacterial Integrons Sulfonamides Tetracycline Resistance Tetracyclines Waste Disposal, Fluid Waste Water |
Type | Article |
Pagination | 1587-1594 |
Volume Number | 569-570 |
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