Effect of multiple freeze–thaw cycles on the detection of anti-SARS- CoV- 2 IgG antibodies
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Date
2021-08-06Author
Shurrab, Farah MAl-Sadeq, Duaa W.
Amanullah, Fathima
Younes, Salma N.
Al-Jighefee, Hadeel
Younes, Nadin
Dargham, Soha R.
Yassine, Hadi M.
Abu Raddad, Laith J.
Nasrallah, Gheyath K.
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Several studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and stored at 4 °C until tested by ELISA. Statistical analysis was performed to test for F/T cycle effect. None of the 10 positive sera became negative after 16 F/T cycles. There was no significant difference in the OD average reading between the first and last F/T cycles, except for one serum with a minimal decline in the OD. The random effect linear regression of log (OD) on the number of cycles showed no significant trend, with a slope consistent with zero (B=−0.0001; 95% CI −0.0008; 0.0006; P-value= 0.781). These results suggest that multiple F/T cycles had no effect on the ability of the ELISA assay to detect SARS-CoV-2 IgG antibodies
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