Effect of multiple freeze–thaw cycles on the detection of anti-SARS- CoV- 2 IgG antibodies
Author | Shurrab, Farah M |
Author | Al-Sadeq, Duaa W. |
Author | Amanullah, Fathima |
Author | Younes, Salma N. |
Author | Al-Jighefee, Hadeel |
Author | Younes, Nadin |
Author | Dargham, Soha R. |
Author | Yassine, Hadi M. |
Author | Abu Raddad, Laith J. |
Author | Nasrallah, Gheyath K. |
Available date | 2021-08-08T10:34:43Z |
Publication Date | 2021-08-06 |
Publication Name | Journal of medical Microbiology |
Identifier | http://dx.doi.org/10.1099/jmm.0.001402 |
Citation | Shurrab et al., Journal of Medical Microbiology 2021;70:001402 |
Abstract | Several studies have investigated the effect of repeated freeze-thaw (F/T) cycles on RNA detection for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, no data are available regarding the effect of repeated F/T cycles on SARS-CoV-2 antibody detection in serum. We investigated the effect of multiple F/T cycles on anti SARS-CoV-2 IgG detection using an ELISA test targeting the nucleocapsid antibodies. Ten positive and 1 negative SARS-CoV-2 IgG sera from 11 participants, in replicates of 5, were subjected to a total of 16 F/T cycles and stored at 4 °C until tested by ELISA. Statistical analysis was performed to test for F/T cycle effect. None of the 10 positive sera became negative after 16 F/T cycles. There was no significant difference in the OD average reading between the first and last F/T cycles, except for one serum with a minimal decline in the OD. The random effect linear regression of log (OD) on the number of cycles showed no significant trend, with a slope consistent with zero (B=−0.0001; 95% CI −0.0008; 0.0006; P-value= 0.781). These results suggest that multiple F/T cycles had no effect on the ability of the ELISA assay to detect SARS-CoV-2 IgG antibodies |
Sponsor | This work was made possible by grant no. RRC-2-032 from the Qatar National Research Fund (a member of the Qatar Foundation). The statements made herein are solely the responsibility of the author(s). G. K. N. acknowledges funds from Qatar University’s internal grant QUERG-CMED-2020-2. S. R. D. and L. J. A. acknowledges the support of the Biomedical Research Program and the Biostatistics, Epidemiology, and Biomathematics Research Core, both at Weill Cornell Medicine-Qatar. |
Language | en |
Publisher | Microbiology Society |
Subject | antibodies detection COVID-19 SARS-CoV- 2 IgG freeze thaw |
Type | Article |
Issue Number | 8 |
Volume Number | 70 |
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COVID-19 Research [802 items ]