Show simple item record

AuthorYaragalla, Srinivasarao
AuthorRajendran, Rajakumari
AuthorAlMaadeed, Jiya Jose Mariam A.
AuthorKalarikkal, Nandakumar
AuthorThomas, Sabu
Available date2021-09-01T10:02:39Z
Publication Date2016
Publication NameMaterials Science and Engineering C
ResourceScopus
URIhttp://dx.doi.org/10.1016/j.msec.2016.04.050
URIhttp://hdl.handle.net/10576/22334
AbstractThe development of functionalized graphene materials concerning health and environmental aspects via green approaches is currently the most recent topic in the field of nanoscience and nanotechnology. Herein, we report the green reduction of graphene oxide (GO) to reduced graphene oxide (RGO) using grape seed extract (GSE). Structural properties of the prepared RGO were investigated using Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, thermogravimetric analysis (TGA), UV-Visible spectroscopy and X-ray diffraction analysis. These all characterization techniques clearly revealed that the RGO has been successfully prepared. Moreover, the average thickness (4.2 nm) of RGO layers was also confirmed by transmission electron microscopy (TEM). Optical properties such as band gap and photoluminescence of the synthesized RGO were evaluated. The band gap of RGO was found to be 3.84 eV and it showed emission in the visible region. Efficient antimicrobial activity against Escherichia coli and Staphylococcus aureus was observed with 4 ?g ml- 1 & 5 ?g ml- 1 of RGO and also the cell wall damage of these strains has been proved by atomic force microscopy (AFM). The in vitro study of RGO (500 ?g) disclosed the effective anti-proliferative activity (88%) against HCT-116 cell lines. 2016 Elsevier B.V. All rights reserved.
SponsorThe authors would like to express their sincere gratitude to University Grants Commission ( 2061010223 ) (UGC), New Delhi, and Department of Science and Technology (DST-Inspire fellowship) , and DST Nano Mission ( SR/NM/NS-54/2009 ), New Delhi, for their financial support. Special thanks to Centre for Advanced Materials (CAM), Qatar University for supporting this work.
Languageen
PublisherElsevier Ltd
SubjectAtomic force microscopy
Bacteria
Cell culture
Energy gap
Escherichia coli
Fourier transform infrared spectroscopy
Graphene
High resolution transmission electron microscopy
Nanoscience
Optical properties
Reduction
Spectrum analysis
Transmission electron microscopy
Ultraviolet visible spectroscopy
X ray diffraction analysis
Anti-proliferative activities
Characterization techniques
Fourier transform infra red (FTIR) spectroscopy
Functionalized graphene
Grape seed extract
Nanoscience and nanotechnologies
Reduced graphene oxides
Reduced graphene oxides (RGO)
Thermogravimetric analysis
grape seed extract
graphite
oxide
atomic force microscopy
cell proliferation
cell survival
chemistry
cytology
drug effects
erythrocyte
Escherichia coli
HCT 116 cell line
hemolysis
human
infrared spectroscopy
Raman spectrometry
Staphylococcus aureus
transmission electron microscopy
X ray diffraction
Cell Proliferation
Cell Survival
Erythrocytes
Escherichia coli
Grape Seed Extract
Graphite
HCT116 Cells
Hemolysis
Humans
Microscopy, Atomic Force
Microscopy, Electron, Transmission
Oxides
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Staphylococcus aureus
X-Ray Diffraction
TitlePreparation and characterization of green graphene using grape seed extract for bioapplications
TypeArticle
Pagination345-353
Volume Number65
dc.accessType Abstract Only


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record