Effect of Water-Pipe Smoking on the Normal Development of Zebrafish
Date
2021-11-06Author
Zakaria, Zain ZakiAladwi, Shaima Ahmad
Benslimane, Fatiha
Al-Absi, Enas S.
Al-Shafai, Mashael
Yalcin, Huseyin C.
Khalil, Ashraf
Al Moustafa, Ala-Eddin
Al-Asmakh, Maha
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Background: Among all types of tobacco consumption,Water-Pipe Smoking (WPS) is the
most widely used in the Middle East and second-most in several other countries. The effect of WPS
on normal development is not yet fully understood, thus the aim of this study is to explore the acute
toxicity effects of WPS extract on zebrafish larvae. Methods: In this study, we compared the effects of
WPS smoke condensates at concentrations varying from 50 to 200 g/mL on developmental, cardiac,
and behavioural (neurotoxicity) functions. Gene expression patterns of cardiac biomarkers were
also evaluated by RT-qPCR. Results: Exposing zebrafish embryos to 50, 100, 150 and 200 g/mL
WPS for three days did not affect the normal morphology of Zebrafish embryos, as the tail flicking,
behavioural and locomotion assays did not show any change. However, WPS deregulated cardiac
markers including atrial natriuretic peptide (ANP/NPPA) and brain natriuretic peptide (BNP/NPPB).
Furthermore, it induced apoptosis in a dose-dependent manner. Conclusion: Our data demonstrate
that WPS can significantly affect specific cardiac parameters during the normal development of
zebrafish. Further investigations are necessary to elucidate the pathogenic outcome of WPS on
different aspects of human life, including pregnancy.
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