Molecular and biological characterization of influenza A viruses isolated from human fecal samples
Author | Al Khatib, Hebah A. |
Author | Coyle, Peter V. |
Author | Al Maslamani, Muna A. |
Author | Al Thani, Asmaa A. |
Author | Pathan, Sameer A. |
Author | Yassine, Hadi M. |
Available date | 2021-11-29T11:37:00Z |
Publication Date | 2021-09-01 |
Publication Name | Infection, Genetics and Evolution |
Identifier | http://dx.doi.org/10.1016/j.meegid.2021.104972 |
Citation | Hebah A. Al Khatib et. al. Molecular and biological characterization of influenza A viruses isolated from human fecal samples, Infection, Genetics and Evolution, Volume 93, 2021, 104972, ISSN 1567-1348, https://doi.org/10.1016/j.meegid.2021.104972 |
ISSN | 15671348 |
Abstract | Human influenza viruses are occasionally detected in the stools of influenza patients. Objectives: Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission. Methods: Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated. Results: Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors. Conclusion: The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission. |
Language | en |
Publisher | Elsevier |
Subject | Influenza virus Intestinal replication Sialic acid receptors Transmission Virus diversity |
Type | Article |
Volume Number | 93 |
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