عرض بسيط للتسجيلة

المؤلفOla, El Atab
المؤلفGhantous, Crystal M.
المؤلفEl-Zein, Nabil
المؤلفFarhat, Rima
المؤلفAgouni, Abdelali
المؤلفKorashy, Hesham M.
المؤلفDjouhri, Laiche
المؤلفKamareddine, Layla
المؤلفZibara, Kazem
المؤلفZeidan, Asad
تاريخ الإتاحة2022-01-17T08:31:54Z
تاريخ النشر2021-12-24
اسم المنشورEuropean Journal of Pharmacology
المعرّفhttp://dx.doi.org/10.1016/j.ejphar.2021.174701
الاقتباسOla El Atab, Crystal M. Ghantous, Nabil El-Zein, Rima Farhat, Abdelali Agouni, Hesham M. Korashy, Laiche Djouhri, Layla Kamareddine, Kazem Zibara, Asad Zeidan, Involvement of caveolae in hyperglycemia-induced changes in adiponectin and leptin expressions in vascular smooth muscle cells, European Journal of Pharmacology, 2021, 174701, ISSN 0014-2999, https://doi.org/10.1016/j.ejphar.2021.174701.
الرقم المعياري الدولي للكتاب00142999
معرّف المصادر الموحدhttps://www.sciencedirect.com/science/article/pii/S0014299921008578?v=s5
معرّف المصادر الموحدhttp://hdl.handle.net/10576/25671
الملخصHyperglycemia exerts various harmful effects on the vasculature. Studies have shown an association between the levels of the adipokines leptin and adiponectin (APN) and vascular complications in diabetes mellitus. The aim of our study was to investigate the molecular mechanisms mediated by APN and leptin that are involved in hyperglycemia-induced vascular remodeling, especially at the level of oxidative stress and actin cytoskeleton dynamics. Rat aorta organ culture was used to investigate the effect of hyperglycemia on APN and leptin protein expression in vascular smooth muscle cells (VSMCs) using Western blot analysis and immunohistochemistry. Hyperglycemia lead to a significant increase in APN synthesis in VSMCs, mainly through caveolae, but this increase failed to provide vascular protection because of the decreased expression of APN receptors, especially AdipoR2, which was assessed by qPCR. In addition, hyperglycemia significantly upregulated leptin expression in VSMCs through caveolae and the RhoA/ROCK pathway. These variations lead to a marked increase in reactive oxygen species (ROS) production, detected by dihydroethidium (DHE) staining, and in NADPH oxidase type 4 (Nox4) expression. Moreover, Nox4 mediated the synthesis of APN in hyperglycemia in VSMCs. Finally, hyperglycemia activated the RhoA/ROCK pathway and subsequently induced the polymerization of globular actin (G-actin) into filamentous actin (F-actin), decreasing the G/F-actin ratio. Taken together, these data show that hyperglycemia increases oxidative stress and changes actin cytoskeleton dynamics in the aorta via caveolae, favoring vascular remodeling.
اللغةen
الناشرElsevier
الموضوعHyperglycemia
Caveolae
Cyclophilin A
Leptin
Adiponectin
Vascular smooth muscle remodeling
العنوانInvolvement of caveolae in hyperglycemia-induced changes in adiponectin and leptin expressions in vascular smooth muscle cells
النوعArticle
dc.accessType Abstract Only


الملفات في هذه التسجيلة

الملفاتالحجمالصيغةالعرض

لا توجد ملفات لها صلة بهذه التسجيلة.

هذه التسجيلة تظهر في المجموعات التالية

عرض بسيط للتسجيلة