Show simple item record

AuthorPierluigi, Barbieri
AuthorZupin, Luisa
AuthorLicen, Sabina
AuthorTorboli, Valentina
AuthorSemeraro, Sabrina
AuthorCozzutto, Sergio
AuthorPalmisani, Jolanda
AuthorDi Gilio, Alessia
Authorde Gennaro, Gianluigi
AuthorFontana, Francesco
AuthorOmiciuolo, Cinzia
AuthorPallavicini, Alberto
AuthorRuscio, Maurizio
AuthorCrovella, Sergio
Available date2022-01-25T06:37:19Z
Publication Date2021-07-31
Publication NameEnvironmental Research
Identifierhttp://dx.doi.org/10.1016/j.envres.2021.111200
CitationBarbieri, P., Zupin, L., Licen, S., Torboli, V., Semeraro, S., Cozzutto, S., Palmisani, J., Di Gilio, A., de Gennaro, G., Fontana, F., Omiciuolo, C., Pallavicini, A., Ruscio, M., & Crovella, S. (2021). Molecular detection of SARS-CoV-2 from indoor air samples in environmental monitoring needs adequate temporal coverage and infectivity assessment. Environmental research, 198, 111200. https://doi.org/10.1016/j.envres.2021.111200
ISSN00139351
URIhttps://www.sciencedirect.com/science/article/pii/S0013935121004941
URIhttp://hdl.handle.net/10576/25910
AbstractThe relevance of airborne exposure to SARS-CoV-2 in indoor environments is a matter of research and debate, with special importance for healthcare low-risk settings. Experimental approaches to the bioaerosol sampling are neither standardized nor optimized yet, leading in some cases to limited representativity of the temporal and spatial variability of viral presence in aerosols. Airborne viral viability moreover needs to be assessed. A study has been conducted collecting five 24-h PM10 samples in a COVID-19 geriatric ward in late June 2020, and detecting E and RdRp genes by RT-qPCR with a Ct between 36 and 39. The viral RNA detection at Ct = 36 was related to the maximal numerosity of infected patients hosted in the ward. Lacking a direct infectivity assessment for the collected samples an experimental model has been defined, by seeding twelve nasopharyngeal swab extracts from COVID-19 positive patients on Vero E6 cells; only the four extracts with a viral load above E+10 viral copies (approximately Ct<24) have been able to establish a persistent infection in vitro. Therefore, the cytopathic effect, a key feature of residual infectivity, could be considered unlikely for the environmental PM10 samples showing amplification of viral RNA at Ct = 36 or higher. A standardization of airborne SARS-CoV-2 long-term monitoring and of environmental infectivity assessment is urgently needed.
SponsorThe project is approved by IRCCS Burlo Garofolo (Trieste, Italy) RC 47/20 and partially funded by the “Ricerca-Corrente” RC 15/17, RC 03/20 from IRCCS Burlo Garofolo (Trieste, Italy) and Italian Ministry of Health (Italy).
Languageen
PublisherElsevier
SubjectSARS-CoV-2
Indoor air
Low-risk healthcare unit
RT-qPCR
Residual infectivity
TitleMolecular detection of SARS-CoV-2 from indoor air samples in environmental monitoring needs adequate temporal coverage and infectivity assessment
TypeArticle
Volume Number198
dc.accessType Open Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record