Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat
Author | Das, Sandra Concepcion |
Author | Varadharajan, Kavitha |
Author | Shanmugakonar, Muralitharan |
Author | Al-Naemi, Hamda A. |
Available date | 2022-02-27T08:29:30Z |
Publication Date | 2021-08-10 |
Publication Name | Frontiers in Pharmacology |
Identifier | http://dx.doi.org/10.3389/fphar.2021.663048 |
Citation | Das SC, Varadharajan K, Shanmugakonar M and Al-Naemi HA (2021) Chronic Cadmium Exposure Alters Cardiac Matrix Metalloproteinases in the Heart of Sprague-Dawley Rat. Front. Pharmacol. 12:663048. doi: 10.3389/fphar.2021.663048 |
Abstract | The aim of this study was to evaluate the role of chronic cadmium exposure in modulating cardiac matrix metalloproteinases (MMPs) in the heart of rats. Adult male Sprague-Dawley rats were exposed to 15 ppm CdCl2 in drinking water for 10 weeks followed by withdrawal of cadmium treatment for 4 weeks. Following the completion of the treatment, gene expression of inflammatory mediators (IL-1β, IL-6, IL-10, TNF-α and NF-κB), protein expression of MMP-2, MMP-9 and their respective inhibitors- TIMP-1 and TIMP-2, and gelatinolytic activity of MMP-2 and MMP-9 were determined. At the protein level, cadmium incites a differential effect on the expression and activity of gelatinases and their endogenous inhibitors in an exposure-dependent manner. Results also show that the administered cadmium dose elicits an inflammatory response until week 10 that slightly diminishes after 4 weeks. This study provides evidence of cadmium-induced imbalance in the MMP-TIMP system in the cardiac tissue. This imbalance may be mediated by cadmium-induced inflammation that could contribute to various cardiovascular pathologies. |
Language | en |
Publisher | Frontiers Media |
Subject | cadmium cardiac inflammatory cytokines MMP TIMP |
Type | Article |
Volume Number | 12 |
ESSN | 1663-9812 |
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