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المؤلفAhmady, Elmira
المؤلفBlais, Alexandre
المؤلفBurgon, Patrick G.
تاريخ الإتاحة2022-03-13T08:28:40Z
تاريخ النشر2021-03-01
اسم المنشورCells
المعرّفhttp://dx.doi.org/10.3390/cells10030615
الاقتباسAhmady, E.; Blais, A.; Burgon, P.G. Muscle Enriched Lamin Interacting Protein (Mlip) Binds Chromatin and Is Required for Myoblast Differentiation. Cells 2021, 10, 615. https://doi.org/10.3390/ cells10030615
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85103862812&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/28039
الملخصMuscle‐enriched A‐type lamin‐interacting protein (Mlip) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report Mlip’s direct interaction with chromatin, and it may function as a transcriptional co‐factor. Chromatin immunoprecipitations with microarray analysis demonstrated a propensity for Mlip to associate with genomic regions in close proximity to genes that control tissue‐specific differentiation. Gel mobility shift assays confirmed that Mlip protein complexes with genomic DNA. Blocking Mlip expression in C2C12 my-oblasts down‐regulates myogenic regulatory factors (MyoD and MyoG) and subsequently significantly inhibits myogenic differentiation and the formation of myotubes. Collectively our data demonstrate that Mlip is required for C2C12 myoblast differentiation into myotubes. Mlip may exert this role as a transcriptional regulator of a myogenic program that is unique to amniotes.
راعي المشروعThis research was funded by an Operating grant from Canadian Health Research Institute and Heart and Stroke Foundation of Canada to PGB (CIHR grant #82748 & 119470; HSFO #0007) and A.B. (CIHR grant #119458). Ontario Graduate Student Science and Technology (OGSST) provided a stipend to E.A.
اللغةen
الناشرMDPI
الموضوعChIP
Mlip
Skeletal muscle
العنوانMuscle enriched lamin interacting protein (Mlip) binds chromatin and is required for myoblast differentiation
النوعArticle
رقم العدد3
رقم المجلد10
ESSN2073-4409
dc.accessType Open Access


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