Understanding the binding interaction between phenyl boronic acid P1 and sugars: determination of association and dissociation constants using S?V plots, steady-state spectroscopic methods and molecular docking
Date
2021Author
Melavanki, RaveendraSharma, Kalpana
Yallur, Basappa Chanabasapa
Kusanur, Raviraj
Sadasivuni, Kishor Kumar
Singh, Diksha
Mane, Smita
Katagi, Kariyappa
Pattar, Shridhar V.
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Continuous monitoring of glucose and sugar sensing plays a vital role in diabetes control. The drawbacks of the present enzyme-based sugar sensors have encouraged the investigation into alternate approaches to design new sensors. The popularity of fluorescence sensors is due to their ability to bind reversibly to compounds containing diol. In this study we investigated the binding ability of phenyl boronic acid P1 for monosaccharides and disaccharides (sugars) in aqueous medium at physiological pH 7.4 using steady-state fluorescence and absorbance. P1 fluorescence was quenched due to formation of esters with sugars. Absorbance and fluorescence measurements led to results that indicated that the sugars studied could be ordered in terms of their affinity to P1, as stated: sucrose > lactose > galactose > xylose > ribose > arabinose. In each case, the slope of modified Stern?Volmer plots was nearly 1, indicating the presence of only a single binding site in boronic acids for sugars. Docking studies were carried out using Schrodinger Maestro v.11.2 software. The binding affinity of phenyl boronic acid P1 with periplasmic protein (PDB ID 2IPM and 2IPL) was estimated using GlideScore.
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