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AuthorAl-absi, Enas
AuthorHaj Ali, Nadima
AuthorKhan, Aisha
AuthorKhalili, Makiyeh
AuthorHadwan, Tameem
AuthorAl-mawlawi, Naema
AuthorAbuodeh, Raed
AuthorKhalid Nasrallah, Gheyath
Available date2022-04-13T06:55:12Z
Publication Date2014
Publication NameQatar Foundation Annual Research Conference Proceedings
Resourceqscience
CitationAl-absi, Enas. Haj Ali, Nadima. Khan, Aisha. Khalili, Makiyeh. Hadwan, Tameem. Al-mawlawi, Naema. Abuodeh, Raed. Khalid Nasrallah, Gheyath. (2014) Infection Rates And Phylogenetic Analysis Of Hepatitis G Virus (hgv)/gb Virus C (gbv-c) Among Qatari Blood Donors. Qatar Foundation Annual Research Conference Proceedings 2014: HBOP0582 https://doi.org/10.5339/qfarc.2014.HBOP0582
ISSN2226-9649
URIhttps://doi.org/10.5339/qfarc.2014.HBOP0582
URIhttp://hdl.handle.net/10576/29652
AbstractBackground: A new immerging nonpathogenic single stranded human RNA virus known as GBV-C/HGV was discovered in 1995. Both GBV-C virus and HCV have similar genome, but they replicate in different cell types. The incidence of GBV-C infection has been studied worldwide, however, to our knowledge; no previous studies were conducted in Qatar, thus the objectives of this study are: (i) to determine the rate of GBV-C infection in Qatar among healthy blood donors and liver-diseased patient and (ii) to determine the most predominant GBV-C genotype in Qatar. Methods: 755 blood plasma samples from blood bank (593) and virology section (162) at HMC were collected. RNA was extracted, reversed transcribed, and then subjected for GBV-C detection by nested PCR using primers targeting a 205 bp of the 5' hypervariable untranslated region (5'-UTR) of the GBV-C/HGV. For genotyping, the 5'-UTR PCR product were T/A cloned into pDrive plasmid. The plasmids were transformed into DH5α™, and then plasmids were purified and digested by EcoRI to detect the positive clones. Plasmid were then purified and sequenced. Phylogenetic analysis was conducted by analyzing the 5'-UTR sequence from randomly picked positive clones. The resulted sequences were assembled and analyzed using CLC and MEGA5 software. Results: we found that HGV infection rate among healthy blood donors was 13.5 %, while in liver-diseased patient was 8.6 %. Moreover, there was no significant difference in the GBV-C infection rate among Qatari (13.3%) and Non-Qatari (14.4%) healthy donors. Sequence analysis of 25 5'-UTR PCR amplicons yielded the European genotype (genotype 2) as the most predominant in Qatar. Conclusion: Our results indicate that there is no correlation between GVC- infection rate and other liver-infecting viruses such as HB&CV. Not surprising, genotype 2 was also reported to be dominant in countries surrounding Qatar such as UAE and Kuwait. Finally, we think our results should benefit epidemiologists in the region and may have an impact on the blood screening policy in blood banks.
Languageen
PublisherHamad bin Khalifa University Press (HBKU Press)
SubjectHepatitis G Virus (hgv)/gb
Qatari Blood Donors
Virus C (gbv-c)
TitleInfection Rates And Phylogenetic Analysis Of Hepatitis G Virus (hgv)/gb Virus C (gbv-c) Among Qatari Blood Donors
TypeConference Paper
Issue Number1
Volume Number2014
dc.accessType Open Access


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